カスタムトランスジェニックマウスモデルの作製と維持
効果的な研究は、お客様の案件にとって最も関連性が高いモデルを見つけられるかどうかにかかっています。お客様のプロジェクトにランダムなDNA遺伝子導入技術が必要な場合は、Charles Riverの科学者がトランスジェニックマウスモデルの作製、維持、分析のための統合ソリューションを提供します。
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トランスジェニックマウスとラットのモデルの作製 |
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どのようなサービスを提供していますか。
- マウスとラットの委託繁殖
- CRISPR/Cas9*ゲノム編集
- ES細胞突然変異誘発
- 遺伝子的試験サービス
- 受精（凍結保存とクリーン化を含む）
- ラピッドコロニーデベロップメント
- 動物検疫
- 系統レスキュー
トランスジェニックマウスはどのように取得できますか。
ランダムDNA挿入：DNA遺伝子導入技術により、Charles Riverのマイクロインジェクション専門家は、お客様の研究室またはCharles Riverの業界パートナーが設計して準備したDNAベクターを接合体の前核に直接インジェクトし、わずか3〜4か月で、ファウンダーがいるトランスジェニックマウスモデルにまさに生命を吹き込みます。
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Package I (North America)
- DNA or BAC injection into one embryo
- Reimplantation into foster females
- Husbandry and weaning
- Sample collection for genetic characterization in founder mice
- Health report
- Delivery of founder mice
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Package II (North America)
- Package I services
- Breeding to F1 generation
- Sample collection and screening of up to 50 mice
- Delivery of heterozygous F1 mice
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Europe
With DNA transgenesis technology, our microinjection experts inject the DNA vector designed and prepared by PHENOMIN-ICS directly into the pronuclei of zygotes, effectively bringing your custom transgenic mouse models to life with founders available in as few as three to four months. As with CRISPR/Cas9*, a large genetic background panel for mice is available. Download our technical sheet
Frequently Asked Questions (FAQs) about Transgenic Mice
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What is a transgenic mouse?
The term "transgenic mouse model" is sometimes used to describe all genetically modified mice, and has sometimes been confused with knockin mice. Random transgenic mice are models that have the DNA material randomly inserted into the genome (a trans-gene) whereas with knockin and targeted transgenic mice the process is targeted and the desired gene is inserted into a specific locus via CRISPR/Cas9* or homologous recombination. This locus could be to mimic expression using an endogenous gene’s promoter, or be stable expression of the transgene from a safe location.
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Which genetic background should I choose?
Our team of scientists will work with you to determine the best model creation approach to suit your research, and can guide your development and selection from our large panel of available mouse genetic backgrounds (C57BL/6N, 129S2/SvPas, FVB/N, etc.).
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What are some of the considerations of using transgenic mice?
When the DNA is integrated, it could be placed under its own (strong) promoter, leading to high levels of expression (good for disease models) and an earlier or more robust phenotype. However, as the desired gene is placed randomly in the host genome, the new transgenic mouse model may not be as relevant a model of human disease. Characterization would be needed to determine how many copies of the transgene integrated and where they inserted. For further advice on using knockin, knockout, and random or targeted transgenic mice, please consult our scientific team. For further information, please visit the International Society for Transgenic Technologies (ISTT).
*CRISPR-Cas9 used under licenses granted, and pending US and international patents from The Broad Institute and ERS Genomics Limited.
Video: Considerations for Creating Knockout Mice and Other Transgenic Animal Models
Related Blogs
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New Gene Editing Tools, Fewer Animals?
The use of CRISPR/Cas9 to edit genomes can lead to reductions in the number of mice and rats used in research. "In individual studies, there is a great opportunity for reduction," says one scientist. Learn why she calls for "an increase in sophistication in genome editing... [and] in the sensitivity and reproducibility of in vivo phenotyping tests." Read the full story
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How CRISPR Accelerates Research
CRISPR-Cas9 enables transgenic mice to be generated more quickly and more precisely than ever. Classical methods of genome editing in rodent models are effective, but slow. Using CRISPR/Cas9 to selectively introduce DNA damage and let cells repair themselves naturally reduces the timeline from months or years, to a matter of weeks. Read the full story