JAX™ NSG® Mouse Variant Portfolio

NSG® mouse model variants are the most highly immunodeficient mice and the models of choice for cancer xenograft modelling, stem cell biology, humanised mice, and infectious disease research. Charles River is the exclusive distributor of JAX™ Mice in Europe.

 

Comparison of NSG® Mouse Model Variants

Full Nomenclature NOD.Cg-PrkdcSCID
Il2rgtm1Wjl/SzJ
NOD.Cg-PrkdcSCID
Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)
1Eav/MloySzJ
NOD.Cg-Rag1tm1Mom
Il2rgtm1Wjl/SzJ
Breeding Location Charles River France, Germany, Japan, and UK Jackson Laboratories USA
Available as import via Charles River
Charles River UK
Stock Number Strain code: 614 JAX™ strain code: 013062 Strain code: 688
Branded or Common Name NSG® (branded name), NOD SCID gamma NSGS, NOD SCID gamma Il3- GM-SF (NSG-SGM3) NRG, NOD Rag gamma
Mature B Cells Absent Absent Absent
Mature T Cells Absent Absent Absent
Dendritic Cells Defective Defective Defective
Macrophages Defective Defective Defective
Natural Killer Cells Absent Absent Absent
Complement Absent Absent Absent
Leakiness Very Low Absent Absent
Irradiation Tolerance Low Low High
Lymphoma Incidence Low Low Low
Benefits • Engrafts the widest range of solid and hematological cancers, including ALL and AML

• Most sensitive host for cancer stem cells when compared to NOD SCID or nude mice

• Longer lifespan than NOD SCID; supports long-term engraftment studies and capabilities; >89 weeks median
•  Increased CD4+ FoxP3+ regulatory T cell population after CD34+ humanization

• Enhances human myelopoiesis and terminal differentiation after CD34+ humanization

• Increased efficiency of engrafting human acute myeloid leukemia (AML)
•  Long-term multilineage hematopoeitic stem cell repopulation similar to NSG mice

• Engrafts human PBMC without irradiation similar to NSG

• Engrafts a wide range if solid and hematological cancers
Considerations •  No thymic lymphomas, can be used for long & short-term experiments

•  Sensitive to irradiation
•  Compromised human stem cell regeneration

•  Suppression of human erythropoiesis

•  Reduction of human B-lymphopoiesis
• No thymic lymphomas, can be used for long & short-term experiments

•  Requires higher dose of irradiation to obtain human HSC engraftment
References Ishikawa et al. 2005;  
Shultz et al. 2005
Nicolini et al. 2004;
Wunderlich et al. 2010;
Billerbeck et al. 2015
Pearson et al. 2008;
Brehm et al. 2010;
Maykel et al. 2014

 

Charles River is now able to offer importation of several additional lines of JAX™ mice including NSG® variants for humanisation, immuno-oncology, immunology, and infectious disease studies.

 

Name & Stock Number NSG-HLA-A2.1 (009617)
NSG-HLA-A2/HHD (014570)
DR1 (012479)
DR4 (017637)
NSG B2m (010636)
NSG-(KbDb)null (023848)
Branded or common name HLA Class I-A2 Transgenics HLA Class II Transgenics MHC Class I-null NSG™
Mature B cells Absent Absent Absent
Mature T cells Absent Absent Absent
Dendritic cells Defective Defective Defective
Macrophages Defective Defective Defective
Natural killer cells Absent Absent Absent
Complement Absent Absent Absent
Leakiness Low Low Low
Irradiation tolerance Low Low Low
Lymphoma incidence Low Low Low
Benefits •  High engraftment of HLA-A2-restricted immune cells after CD34+ humanization

•  Enable functional CD4+ T cell responses to viral infection after CD34+ humanization (014570)
•  Useful for transplantation studies in the absence of xeno-GVHD

•  Develop allo-GVHD post-engraftmentof DR4-negative CD4+ T cells (017637)
•  Resistant to xeno-GVHD (010636)

•  Attenuated xeno-GVHD development post-hPBMC transplantation (023848)

•  High hCD45+ cell engraftment (023848)

•  Useful for studying mechanisms for xeno-GVHD
Considerations •  No thymic lymphomas - can be used for long-term experiments

•  Sensitive to irradiation
•  Reduced CD45+ cell engraftment compared to NSG

•  Higher proportion of mice with no CD45+ cell engraftment as compared to NSG (017637)
•  Reduced survival post hCD34+ cell transplantation compared to NSG mice (023848)    
References Shultz et al. 2010 Covassin et al. 2011 Covassin et al. 2013