Our Fast, Objective, Validated Method for Microbial Identification
Starting with a pure isolate of bacteria, yeast, or mould, we perform a DNA extraction, amplification, and sequencing of the amplicon using state-of-the-art fluorescent di-deoxy terminator cycle chemistry. The extension products are then separated on an automated DNA sequencer, and the data is compared to our comprehensive and proprietary validated library of known sequences.
Even if a species-level determination can't be made, the organism's stable 16S sequence can be used for future isolate comparisons. In addition, we also supply a wide range of specialized data generation and analysis services to facilitate tracking and trending of your individual sites or at a global level. It is superior to current phenotypic procedures which can take days even weeks to complete and whose results are often inaccurate and unreliable.
A validated library and reporting process is critical when you choose to sequence your microorganism and analyze the data with an optimized, cGMP-compliant process. Once the DNA sequence is analyzed, it is compared to a relevant, validated library of known organisms, and an identification report is generated and interpreted.
Available Microbial Identification Services
This revolutionary system identifies microbial samples based on DNA, providing accurate, reproducible results without the need for special growth requirements. Rapid technology can identify even the most difficult samples with short turnover times. Microbial identification is based on sequence data of the 16S rRNA gene. This gene is universally found in all bacteria and is considered one of the best genes for use in new species identification. With the system used at Microgenetix, we offer a service whose accuracy and speed is better than any other conventional microbial identification techniques. We are able to identify bacteria that are non-viable, difficult to grow, biochemically inert, and difficult or impossible to identify using conventional methods.
Identification of both yeasts and filamentous fungi begins with DNA extraction from pure isolates. The process normally requires very few cells. Standardized growth conditions are not required and the technology works equally well with healthy, damaged, and non-viable cells.
Reagent Ordering Information
PrepMan Ultra DNA Preparation Reagent may be ordered directly from Microgenetix. The prepman buffer is available in the following pack formats:
- 5x200µl in 2ml transport tubes – Cat Number- MGXPMAN-005
- 10x200µl in 2ml transport tubes – Cat Number- MGXPMAN-010
- 50x200µl in 2ml transport tubes – Cat Number- MGXPMAN-050
- 100x200µl in 2ml transport tubes – Cat Number- MGXPMAN-100