Avian Testing Services
Charles River offers a wide array of avian diagnostic and regulatory testing services, including serological profiling, virus isolation and vaccine testing. Protocols are available for standard testing methods and customized services.
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Multiplexed Fluorometric Immunoassay® (MFIA®) Tests
MFIA® testing can only be performed on SPF and research flocks and is performed on individual chicken samples. Please provide 0.05 ml per sample.
Test Description Test # 14 Panel MFIA® Test
(HEV, AE, ANV, PMV-2, Reo, F. Pox, IBV, IBDV, ALV-A, ALV-B, ALV-J, NDV, AI & ILT)15100327 22 Panel MFIA® Test
(14 panel and Adeno Grp I, Adeno Grp III, Rota, MD, Rev, MG, MS, & Salmonella Pullorum-Gallinarum)15100329 Single Plex - Individual analytes as listed above Call for more info -
Serum Plate Agglutination Tests
Please provide 0.1 ml serum per test.
Pathogen Test # Mycoplasma Gallisepticum 15100012 Mycoplasma Synoviae 15100013 Salmonella Pullorum-Gallinarum 15100016 -
HA-HI Assays
Please provide 0.025 ml serum per hemagglutination (HA) and hemagglutination-inhibition (HI) test.
Pathogen Test # Adenovirus Group III (EDS 76) 15100019 Avian Influenza Type A 15100020 Avian Paramyxovirus Type 2 15100029 Avian Paramyxovirus Type 3 15100030 Human Influenza A/PR/8/34 15100098 Infectious Bronchitis (Ark.) 15100021 Infectious Bronchitis (Conn.) 15100022 Infectious Bronchitis (DE-072) 15100025 Infectious Bronchitis (JMK) 15100023 Infectious Bronchitis (Mass.) 15100024 Mycoplasma Gallisepticum 15100026 Mycoplasma Synoviae 15100027 Newcastle Disease 15100028 -
Serum Neutralization Tests in SPF Eggs
Please provide 2.0 ml serum per test.
Pathogen Test # Avian Adenovirus (CELO-Phelps) 15100032 Avian Encephalomyelitis 15100033 Infectious Bronchitis (Ark.) 15100034 Infectious Bronchitis (Conn.) 15100035 Infectious Bronchitis (JMK) 15100036 Infectious Bronchitis (Mass.) 15100037 Infectious Bursal Disease (Gumboro) 15100038 Infectious Bursal Disease (Variant E) 15100039 Infectious Laryngotracheitis 15100040 Newcastle Disease 15100041 -
Agar Gel Precipitin Tests
Please provide 0.050 ml serum per test.
Pathogen Test # Avian Adenovirus Group I 15100042 Avian Adenovirus Group II (HEV) 15100048 Avian Encephalomyelitis 15100043 Avian Reovirus (Viral Arthritis) 15100045 Avian Rotavirus 15100046 Fowl Pox 15100047 Infectious Bursal Disease (Gumboro) 15100050 Infectious Bursal Disease Sero Type 2 15100052 Infectious Laryngotracheitis 15100051 Influenza 15100044 Marek’s Disease 15100053 Newcastle Disease 15100054 Reticuloendotheliosis 15100055 -
Microneutralization Tests
Please provide 0.25 ml serum per test.
Pathogen Test # Avian Adenovirus (each sample tested individually for 12 serotypes) 15100056 Avian Reovirus 15100057 RSV-A 15100083 RSV-B 15100084 Avian Leukosis Subgroup J 15100085 -
IFA Testing
Please provide 0.1 ml per serum sample. Chicken sera only.
Pathogen Test # Chicken Anemia Virus 15100075 Avian Nephritis Virus 15100078 Avian Reovirus 15100080 Reticuloendotheliosis Virus 15100081 -
9 CFR Testing
Please provide the quantities and volumes listed below.
9CFR # Test Description Test # 113.26 Detection of Viable Bacteria and Fungi Except in Live Vaccine* 15100071 113.27 113.27 Detection of Extraneous Viable Bacteria and Fungi in Live Vaccine (10 x 0.5 ml) 15100070 113.28 Detection of Mycoplasma Contamination (1.5 ml) 15100072 113.30 Detection of Salmonella Contamination (2.5 ml of cells or 11 ml of virus) 15100073 113.31 Detection of Avian Lymphoid Leukosis
(A p27 ELISA is performed in place of CF; 5 ml of cells or 1 ml of virus with 1 ml of antiserum)15100059 113.34 Detection of Hemagglutinating Viruses (2.5 ml) 15100067 113.37 Detection of Pathogens by the Chicken Embryo Inoculation Test** 15100068 113.46 Detection of Cytopathogenic and/or Hemadsorbing Agents (2.5 ml) 15100069 * Each final product serial: 10 x 2.5 ml. Cells & cell lines and ingredients of animal origin: 1 x 22 ml.
** 5 ml of cells or 1 ml of virus with 10 ml of antiserum. -
PCR Tests
Gel-Based PCR
Please provide 0.50 ml per sample.
Pathogen Test # Chicken Anemia Virus 15100076 Mycoplasma synoviae and gallisepticum 15100061 Reticuloendotheliosis (REV) 15100062 Endpoint & Real-time PCR
Cloacal swabs or other samples upon request.
Pathogen Test # Avian Adenovirus Group I, Serotypes 1–12 15100099 Avian Adenovirus Group III (EDS) 15100309 Avian Encephalomyelitis 15100101 Avian Reovirus 15100105 Avian Leukosis Subgroup A 15100102 Avian Leukosis Subgroup B 15100103 Avian Leukosis Subgroup J 15100104 Chicken Anemia Virus (Minimum of 10 samples) 15100106 Mycoplasma synoviae and gallisepticum 15100107 Reticuloendotheliosis 15100108 Salmonella pullorum 15100109 -
ELISA
Antibody Detection
Please provide 0.1 ml chicken serum per test. There is a 30 sample minimum per pathogen for ELISA testing.
Pathogen Test # Avian Encephalomyelitis 15100003 Avian Influenza 15100004 Avian Reovirus 15100006 Avian Rhinotracheitis Virus 15100007 Chicken Anemia Virus 15100008 Infectious Bronchitis 15100009 Infectious Bursal Disease 15100010 Infectious Laryngotracheitis 15100011 Newcastle Disease 15100014 Antigen Detection
Please provide cloacal swabs, albumen, or 0.5 ml of supernatant from passaged CEFs.
Pathogen Test # Avian Leukosis (p27 antigen detection) 15100005 -
Other Tests
Please provide 1.0 ml per sample.
Testing Service Test # Avian Encephalomyelitis Flock Susceptibility 15100082 COFAL (Complement Fixation for Avian Leukosis) 10-day test 15100060 Salmonella pullorum microtiter 15100017 Vaccine Potency Test - Single Vaccine 15100064 Virus Titration 15100065 Virus Isolations 15100066