Primary Chicken Embryo Cells

We routinely prepare small and bulk production lots of chicken embryo fibroblasts (CEF), chicken embryo kidney cells (CEKC), chicken embryo liver cells (CELC) and chicken embryo dermal cells (CED). Custom cell preparations are also available.

Cells are prepared fresh each day to order using our own SPF chicken embryos and media containing irradiated serum from countries with a negligible risk of bovine spongiform encephalopathy (BSE). We offer three quality groups of cells: standard, chicken anemia virus (CAV) negative, and CAV-negative group-specific (GS) antigen-free cells which are C/O and not resistant to any of the Avian Leukosis Virus subgroups.

Standard Cells Material #*
Fibroblasts 10100795
Kidney Cells 10100797
*400 million cells or greater per 50 ml vial.
CAV-Negative Cells Material #
Fibroblasts* 10100807
Kidney Cells* 10100810
Dermal Cells** 10100803
NOTE: Flock source is CAV-negative.
*400 million cells or greater per 50 ml vial.
**1 billion cells or greater per 125 ml vial. Requires a 2 week notice prior to ship date.
CAV-Negative GS Antigen-Free Cells Material #*
Fibroblasts (Formerly Genetic Specific) 10100808
Kidney Cells (Formerly Genetic Specific) 10100811
Liver Cells (Formerly Genetic Specific) 10100814
*400 million cells or greater per 50 ml vial.

Preparation of Cells

  • Protocol for Harvesting and Preparing Chicken Embryo Cells

    Cells are prepared in a virus-free cell lab in which only tissue culture media and cell preparation is performed and employee access is restricted. Cells are prepared under certified (Class A) laminar flow hoods using aseptic technique throughout the harvesting process. Batch records for all the steps in the process, including cell batch records, documentation of the sources, media components and cell performance/viability, are maintained. After the cells are harvested, a sample is seeded into tissue culture flasks and maintained for 48 hours to verify cell performance. A sample of the cells is also taken, plated onto blood agar and then incubated for 48 hours. The plates are checked after 24 and 48 hours of incubation. We believe that checking after 24 hours assures our customers that bioburden is not likely present prior to our customers receiving the cells. If a bioburden issue occurs, customers are notified immediately.

Tissue Culture Media

Basal Medium Eagle (BME) containing irradiated fetal calf serum (FCS) and/or newborn calf serum (NBCS) sourced from negligible risk BSE countries. Sold in 1L quantities as 2 x 500 ml. Custom media is also available upon request.

Media Type Material #
2.5% (2.5% FCS; for use with CEF) 10100802
5% (1% FCS & 4% NBCS; for use with CEF) 10100799
8% (2% FCS & 6% NBCS; for use with CEK & CEL) 10100801

Laboratory Products & Testing Services 2021 Price List