Glycoprotein Characterization Services
Charles River offers a comprehensive panel of assays for the characterization of glycoproteins, including primary, secondary, tertiary, higher order structures, and protein binding (affinity) methods. Services we offer include:
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PRIMARY STRUCTURE
- LC-MS/MS Peptide Mapping
- Amino Acid Sequence Verification
- Post Translational Modification Mapping
- N- and O-Linked Glycosylation Mapping
- Oxidation, Deamidation, Glycation Mapping
- LC-MS Intact Molecular Weight and Antibody-Drug-Conjugate Analysis
- N-Terminal Sequence for N-terminal truncations
- Amino Acid Analysis and Extinction Coefficient Determination
- LC-MS/MS Peptide Mapping
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SECONDARY STRUCTURE
- LC-MS Disulfide Bridge Mapping
- Circular Dichroism (CD)
- Fourier Transform Infrared (FTIR)
- Intrinsic Tryptophan Fluorescence (ITF)
- Differential Scanning Calorimetry (DSC)
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ISOFORM & PURITY ANALYSIS
- Capillary Electrophoresis:
- cIEF
- CE-SDS
- Zone- electrophoresis
- Imaging capillary electrophoresis (ICE)
- SDS PAGE, and IEF
- LC-MS/MS Impurity and Degradant Characterization
- HPLC Methods such as RP HPLC, IEX
- Capillary Electrophoresis:
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AGGREGATION, PARTICLE SIZE & HIGHER-ORDER STRUCTURES
- Size Exclusion HPLC UV/MALLS
- Analytical Ultracentrifugation (AUC)
- SDS-PAGE
- Dynamic Light Scattering (DLS)
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ACTIVITY
- Protein Binding Affinity via Surface Plasmon Resonance (SPR)
- ELISA for Clone Selection programs through cGMP lot release
- Enzymatic / Colorimetric Assays
- Cell-based assays