Protein Glycosylation Analysis

Glycosylation is the enzymatic process that attaches glycans to proteins, or other organic molecules. Glycosylation of some proteins has a great impact on their structures and functions, and interactions of protein‐linked glycans with carbohydrate‐specific proteins (lectins) modulate many important biological processes. Charles River offers an expert panel of services for the characterization of glycosylation. We specialize in comparability programs that may be used for manufacturing/process changes and for comparisons of originator vs. biosimilar products.

  • Glycosylation Site Occupancy and Mapping via Peptide Mapping LC-MS/MS
  • N-Linked Oligosaccharide Profile via LC-MS/MS
  • O-Linked Oligosaccharide Profile via LC-MS/MS
  • Quantitative Monosaccharide Analysis
  • Quantitative Sialic Acid Determination
  • Glycan Structural Characterization / Confirmation via MSn
  • Glycan Profile / Isoform via cIEF
  • Exoglycosidase Glycan Sequence / Mapping

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