Overview

Charles River offers a comprehensive panel of assays for the characterization of glycoproteins, including primary, secondary, tertiary, higher order structures, and protein binding (affinity) methods. Services we offer include:

  • Primary Structure
    • LC-MS/MS Peptide Mapping
      • Amino Acid Sequence Verification
      • Post Translational Modification Mapping
      • N- and O-Linked Glycosylation Mapping
      • Oxidation, Deamidation, Glycation Mapping
    • LC-MS Intact Molecular Weight and Antibody-Drug-Conjugate Analysis
    • N-Terminal Sequence for N-terminal truncations
    • Amino Acid Analysis and Extinction Coefficient Determination
  • Secondary Structure
    • LC-MS Disulfide Bridge Mapping
    • Circular Dichroism (CD)
    • Fourier Transform Infrared (FTIR)
    • Intrinsic Tryptophan Fluorescence (ITF)
    • Differential Scanning Calorimetry (DSC)
  • Isoform and Purity Analysis
    • Capillary Electrophoresis:
      • cIEF
      • CE-SDS
      • Zone- electrophoresis
      • Imaging capillary electrophoresis (ICE)
    • SDS PAGE, and IEF
    • LC-MS/MS Impurity and Degradant Characterization
    • HPLC Methods such as RP HPLC, IEX
  • Aggregation, Particle Size, and Higher- Order Structures
    • Size Exclusion HPLC UV/MALLS
    • Analytical Ultracentrifugation (AUC)
    • SDS-PAGE
    • Dynamic Light Scattering (DLS)
  • Activity
    • Protein Binding Affinity via Surface Plasmon Resonance (SPR)
    • ELISA for Clone Selection programs through cGMP lot release
    • Enzymatic / Colorimetric Assays
    • Cell-based assays