Plasmid and Viral Vector Gene Therapy Products
Explore our plasmid and viral vector gene therapy products offered by Vigene Biosciences, a Charles River company:
Have a question regarding plasmid and viral vector products?
Need Custom Viral Vector Production? We can help you produce custom viral vectors tailored to your specific needs. Learn more about custom viral vector packaging services.
Frequently Asked Questions (FAQs) for Plasmid and Viral Vector Products
Which serotype should I use?
Specificity is determined by the viral capsid proteins, so it is important to select the correct AAV serotype to ensure optimal gene delivery. The table below lists our recommended AAV serotypes for different target tissues. If you are unsure which AAV serotype will work best for your system, we recommend that you try out our AAV GFP Testing Kit. The testing kit contains the following viruses all driven by the strong CMV promoter: GFP AAV1, GFP AAV2, GFP AAV5, GFP AAV6, GFP AAV8, GFP AAV9, and GFP AAV-DJ.
Tissue type Recommended AAV Serotypes Muscle AAV1, AAV6, AAV8, AAV9 Liver AAV8, AAV9, AAV-DJ Lung AAV5, AAV6, AAV9 Central Nervous System AAV1, AAV5, AAV8, AAV9, AAV-DJ Retina AAV1, AAV2, AAV5, AAV8 Pancreas AAV8 Kidney AAV2, AAV9 Heart AAV1, AAV8, AAV9
Which promoter should I use?
Please use this table to help you decide which promoter may work best for your experiments.
Tissue type Recommended AAV Serotypes Muscle MCK Liver ALB, TBG Neurons CaMKIIa, CK0.4, CK1.3, Syn, MeCP2, c-Fos, NSE, SST Astrocytes GFAP Oligodendrocytes MBP Retina Rpe65 Pancreas PDX1 Heart aMHC, cTnT Ubiquitous CAG, CMV, EF1a, EFFS, UBC, PGK
- Where can I find more information about biosensors?
What are differences between the different GCaMP biosensors?
The number following GCaMP refers to the generation (3rd, 5th, 6th, or 7th). The letter at the end refers to the characteristics of the variant.
jGCaMP7s: sensitive and slow
jGCaMP7f: fast kinetics
jGCaMP7b: brighter baseline
jGCaMP7c: high contrast
The plasmids were generated by the Douglas Kim lab. For more information about the jGCaMP7 variants, please see their publication.
Where can I find more information about these Parkinson’s Disease Tools?
There are currently no publications for the alpha-synuclein knockdown viral vectors, so please refer to the alpha-synuclein knockdown validation data sheet. There have been a number of publications using the A53T alpha-synuclein overexpression viral vectors. Please note the viral vectors we sell do not belong to the same lot as those published; however, all of our viral vectors have been validated (A53T alpha-synuclein validation data sheet).
Ip, C. W. et al. AAV1/2-induced overexpression of A53T-α-synuclein in the substantia nigra results in degeneration of the nigrostriatal system with Lewy-like pathology and motor impairment: a new mouse model for Parkinson’s disease. Acta Neuropathol Commun 5, 11 (2017).
Koprich, J. B., et al. Towards a Non-Human Primate Model of Alpha-Synucleinopathy for Development of Therapeutics for Parkinson’s Disease: Optimization of AAV1/2 Delivery Parameters to Drive Sustained Expression of Alpha Synuclein and Dopaminergic Degeneration in Macaque. PLoS ONE11, e0167235 (2016).
Koprich, J. B., et al. Expression of human A53T alpha-synuclein in the rat substantia nigra using a novel AAV1/2 vector produces a rapidly evolving pathology with protein aggregation, dystrophic neurite architecture and nigrostriatal degeneration with potential to model the pathology of Parkinson’s disease. MolNeurodegener5, 43 (2010)
Are AAV products safe to use?
Yes, all of our AAV Parkinson’s disease tools are replication-incompetent and can be handled safely in a BSL-1 facility.
Are there any limitations to which ORFs can be shuttled?
If you want to generate viral particles, you must not exceed the packaging capacity of the virus. Please note that the packaging capacity refers to the total insert size between and including the ITRs or LTRs.
AAV cloning capacity: 4.7 kb
Adenovirus cloning capacity: 7.5 kb
Lentivirus cloning capacity: 6 kb
To learn more about our molecular cloning services, click here.
How is ORF shuttled into a different vector?
The ORF cloned into the pENTER adenovirus shuttle vector can be transferred into the other Charles River’s destination vectors through a simple “cut and paste” restriction enzyme digestion and ligation. A combination of two pairs of restriction enzymes, AsiSI/MluI (96%), AsiSI/RsrII (99%) covers 99.5% of all human cDNA ORFs. All human genes can be covered with a few additional restriction enzyme combinations, such as AsiSI/NotI, AscI/RsrII.
When should I use plasmid versus virus for miRNA delivery?
Plasmid miRNA delivery may be sufficient for in vitro work or for conducting pilot studies. However, recombinant viruses are excellent tools for miRNA delivery both in vitro and in vivo. Adenovirus miRNAs in particular are a great choice if you need high transduction efficiency.
- Does Charles River validate shRNA sequences?
What promoters, reporters, and tags do you have available?
By default, expression of our lentivirus ORF cDNA clones are driven by the CMV promoter. C-terminal FLAG and Myc tags are included to allow for easy detection and purification of the overexpressed protein. If you would like to request a custom product, please contact us with your project details.