Explore our Molecular Cloning Services

Learn more about our molecular cloning services offered by Vigene Biosciences, a Charles River company, to see how we can help to accelerate your research discovery programs:

 

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Frequently Asked Questions (FAQs) for Molecular Cloning Services

  • Are there any limitations to which ORFs can be shuttled?

    If you want to generate viral particles, you must not exceed the packaging capacity of the virus. Please note that the packaging capacity refers to the total insert size between and including the ITRs or LTRs.

    • AAV cloning capacity: 4.7 kb
    • Adenovirus cloning capacity: 7.5 kb
    • Lentivirus cloning capacity: 6 kb
  • What information do I need to provide for gene synthesis services?

    For gene synthesis services, please provide the following:

    • Gene ID/Name (specify variant, if applicable) or file containing the desired sequence
    • Name of desired viral vector backbone or name of customer-provided vector
  • What information do I need to provide for mutagenesis services?

    For mutagenesis services, please provide the following:

    • Gene ID/Name (specify variant, if applicable) or file containing the desired sequence
    • Description of sites/base pairs to mutate
    • Name of desired viral vector backbone or name of customer-provided vector
    • If you are sending your own vector for mutating or cloning, please send us at least 5 ug and a sequence/plasmid map
  • Does Charles River validate shRNA sequences?

    We do not validate shRNA sequences designed as a part of the shRNA molecular cloning services. However, we will send you the shRNA plasmids to test. Once you determine which shRNA construct works best for your experiments, we can package your selected shRNA construct into virus.