Overview

With decades of experience running in vitro ADME assays and analyzing the data, we can offer those same in vitro assays and bioanalyses more efficiently to generate ADME data earlier in the drug discovery process. This high-speed process optimization and reproducibility can generate quality ADME data for every compound in your chemical library.

To help streamline the early drug-discovery process, we have successfully validated and deployed automation and IT infrastructure to enable rapid delivery of in vitro ADME results. The workflow enables up to 500 compounds per week to support primary assays:

  • Microsomal stability
  • Permeabililty (Caco-2 and MDCK-MDR1)  
  • Protein binding
  • RBC partitioning (Blood/Plasma ratio)

High-Speed Data Acquisition


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Figure 1: 160 permeability samples analyzed in 30 minutes

Validation Data

Below are manual vs. HT LC-MS/MS and automated liquid handling workflows using new chemical entities (NCEs) and marketed compounds.

  • MICROSOMAL STABILITY ASSAY

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    Figure 2: Microsomal – HT LC-MS automated vs. manual

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    Figure 3: Microsomal stability – HT assay performance across multiple runs

  • CACO-2 PERMEABILITY ASSAY

    caco-2-permeability-consistent-performance.jpg

    Figure 4: Consistent automated assay performance

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    Figure 5: Similar performance UPLC-MS vs. HT-MS (ADDA)

  • PROTEIN BINDING ASSAY

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    Figure 6: Protein binding – HT LC-MS automated vs. manual

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    Figure 7: Protein binding – consistent automated assay performance over time

  • RBC PARTITIONING ASSAY

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    Figure 8: RBC partitioning – HT LC-MS vs. UPLCMS