CFE Model

Charles River conducts contract studies in an established animal model of carrageenan footpad edema (CFE) to test the efficacy of novel therapeutics. This model provides rapid assessment of a compound’s ability to provide anti-inflammatory and analgesic activity. Agents that show activity in this assay include NSAIDs, glucocorticoids, and coxibs (modest).

How can we support your program?

Animals are treated with compound prior to challenge with λ-carrageenan in one hind footpad. The contralateral footpad is injected with saline. Footpad thickness is measured at specified time points. Determination of tissue and/or serum cytokine levels and histologic analysis can also be performed.

Study Endpoints

  • Clinical scores
  • Paw volume measurements (plethysmometer)
  • Tactile allodynia (Von Frey fibers)
  • Thermal hypersensitivity (modified Hargreaves)
  • Histopathology
  • Cytokine analysis
  • Clinical chemistry
  • PK/PD blood analysis