• Serum cytokine analysis
  • Inducer: Anti-CD3 monoclonal antibody
  • Species: Mouse

Administration of certain biotherapies transiently induces immediate pro-inflammatory cytokine release from T cells. This phenomenon is commonly known as antibody-induced cytokine release syndrome. In extreme cases, it can lead to cytokine storm with serious consequences. In order to mimic the phenomenon, antibody (clone 145-2C11) targeting murine CD3 are commonly administered in mice, and level of serum cytokines, such as interferon gamma (INFg) and interleukin 2 (IL-2) are measured.

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At Charles River, we offer the mouse in vivo monoclonal anti-CD3 cytokine release assay to screen effects of immunosuppressive drug candidates.

graph showing measurement of serum IFN-gamma level

Figure 1: In vivo anti-CD3 (a-CD3)-induced cytokine release assay for measurement of serum IFN-gamma level at three hours following antibody injection – Dexamethasone (Dex) was administered orally one hour before antibody injection.