• Antigen: NP-Ficoll conjugate
  • Species: Mouse

Antigens are classified as T cell dependent or T cell independent, according to the requirement of T cell involvement in the mounting of an antibody response. T-independent antigens are typically polysaccharides that cannot be presented via MHC molecules. T-independent immune response is further classified into two types, depending on the nature of the antigen. T-independent type I antigens correspond to mitogenic stimuli such as LPS, CpG, or poly-IC that elicit polyclonal B cell activation via toll-like receptors, whereas T-independent type II antigens are polysaccharides that engage the B cell receptor and thus induce antigen-specific B cell responses.

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Charles River conducts contract studies with T-independent type II antigens like 2,4,6-trinitrophenyl (TNP)-Ficoll to test the efficacy of novel therapeutics in an established model of T-cell independent antibody response. After immunization with TNP-Ficoll, the robustness and long-lasting primary antibody response in mice is followed.

graph showing measurement of antigen-induced serum IgM and IgG antibody levels

Figure 1: TIDAR assay for measurement of antigen-induced serum IgM and IgG antibody levels – The relative level of IgG and IgM is measured following the administration of NP-Ficoll at three different concentrations.