From Single Cell to Neural Networks
Electrophysiology studies are a direct way to measure neuronal function, detect pathological functional abnormalities and analyze changes in spontaneous and evoked electrical signals in response to therapeutics or disease modeling. Up- or down-regulation of synaptic transmission can shed light on cellular and network mechanisms of numerous CNS diseases, including yet not limited to Alzheimer’s disease, Parkinson’s disease, Autism, and Pain. Charles River offers a full range of in vitro and in vivo electrophysiology assays where electrical activity from action potentials or field potentials are amplified and recorded using state-of-the-art equipment.
Electrophysiology studies capabilities:
Patch Clamp Electrophysiology
Patch-clamp electrophysiology provides the highest resolution recordings from a single neuron down to a single channel. Physiological and pharmacological questions can be answered using this technique to understand mechanisms of action. Patch clamp is performed both in vitro and in vivo to evaluate compound effects on single proteins and receptors. These electrophysiology studies are routinely used to evaluate mechanism of action, disease modeling and high throughput ion channel screening for hit discovery in neurodegeneration, pain, seizure and psychiatric disorders.
Multielectrode array is an array of microscopic electrodes distributed over a small surface area. They are spaced to cover the center of a single or multi-well. MEA recordings enable high throughput field potential recordings of brain slices, dissociated tissues, organoids, spheroids or 2-D neuronal cell culture.
Frequently Asked Questions (FAQs) for Electrophysiology Studies
How will electrophysiology studies help my research goals?
Your compound can be evaluated in vivo or in vitro under the most physiologically relevant conditions to probe mechanisms of action questions addressed at probing the effects on specific neurons, receptors, ion channels or neurotransmitter systems. Electrophysiology studies can provide functional abnormalities of neuronal circuitry in genetic or pharmacological models of CNS disorders such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, epilepsy, ataxia, schizophrenia and other psychiatric disorders.
What kind of information do you get electrophysiology studies?
Electrophysiological recordings directly measure endogenous neural function. Spontaneous or evoked electrical events generated by individual neurons or large neuronal populations reflect voltage changes from ligand gated ion channels. These voltage changes tell us important features about a single neuron or neuronal population derived from various endpoints related to amplitude, frequency and waveform morphology.