Mouse Model of Neuropathic Pain Induced by Chemotherapy Agent

Charles River scientists recently characterized the oxaliplatin mouse model’s response to cold allodynia, or pain in response to cooling, using two behavioral tests—the tail immersion/flick test and the acetone cooling test. Cold allodynia occurred during and after oxaliplatin infusion, and is thought to arise from a direct effect of oxaliplatin on peripheral sensory neurons. Review the key findings and select data below.

  • Exposure to oxaliplatin led to persistent cool allodynia in the acetone test, which is a reliable method to test oxaliplatin-induced peripheral neuropathy.
  • Treatment with nerve pain medications, pregabalin and duloxetine, alleviated the allodynia response.
  • There was significant variation within each group (control and treated) in the tail immersion/flick test, suggesting that the tail immersion/flick test is not a reliable method to measure cool allodynia.

    The acetone cooling test is a reliable method to measure cold allodynia in control mice and oxaliplatin-induced pain mouse models.

    chart of acetone test responses over time in oxaliplatin model

    Tail immersion test over time. Significant difference in latency to tail flick response was seen in multiple, but not all, time-points.


    Nerve pain medications helped alleviate cold allodynia in oxaliplatin-treated mice.

    charts of pharmacological reversal of acetone cooling response in oxaliplatin-treated mice by Pregabaline and Duloxetine