Blue Light Exposure Model of Retinal Photoreceptor Degeneration in Mice

Light exposure is a common method of modeling the photoreceptor degeneration seen in human diseases such as retinitis pigmentosa and age-related macular degeneration.

Charles River offers a blue light exposure model that induces retinal damage and cell death to be used as a screen for investigating potential neuroprotective properties of new chemical or biological entities (NCEs/NBEs) prior to subsequent nonclinical testing. With shorter study durations and the ability to ensure relatively uniform exposure and model optimization, this model is a favorable alternative to a genetically engineered model.

Model: Albino Mouse (BALB/c)
Endpoint: Outer nuclear layer thickness measured by:

  • Pathology
  • Optical coherence tomography (OCT)

Electroretinogram (ERG)

Intravitreal Treatment (IVT) Duration of Light Exposure (hours) ONL Thickness (cell layers) ERG Amplitude % change
Saline 6 2.24 >-90%
BDNF 6 11.53 +21%
Saline 24 1.24 >-90%
BDNF 24 10.78 -42%
Baseline n/a 12 0

*460-490nm, 1100 lux

OCT Images

  • Eye A = baseline ONL
  • Eyes B-E = saline IVT
  • Eyes F-I = BDNF IVT
  • Green bar = ONL thickness

Optical coherence tomography images measuring ONL thickness of rat eyes with and without saline intravitreal treatmentOptical coherence tomography images of ONL thickness in rat eyes intravitreally treated with BDNF