Blue Light Exposure Model of Retinal Photoreceptor Degeneration in Mice
Light exposure is a common method of modeling the photoreceptor degeneration seen in human diseases such as retinitis pigmentosa and age-related macular degeneration.
Charles River offers a blue light exposure model that induces retinal damage and cell death to be used as a screen for investigating potential neuroprotective properties of new chemical or biological entities (NCEs/NBEs) prior to subsequent nonclinical testing. With shorter study durations and the ability to ensure relatively uniform exposure and model optimization, this model is a favorable alternative to a genetically engineered model.
Model: Albino Mouse (BALB/c)
Endpoint: Outer nuclear layer thickness measured by:
- Pathology
- Optical coherence tomography (OCT)
Electroretinogram (ERG)
| Intravitreal Treatment (IVT) | Duration of Light Exposure (hours) | ONL Thickness (cell layers) | ERG Amplitude % change |
| Saline | 6 | 2.24 | >-90% |
| BDNF | 6 | 11.53 | +21% |
| Saline | 24 | 1.24 | >-90% |
| BDNF | 24 | 10.78 | -42% |
| Baseline | n/a | 12 | 0 |
*460-490nm, 1100 lux
OCT Images
- Eye A = baseline ONL
- Eyes B-E = saline IVT
- Eyes F-I = BDNF IVT
- Green bar = ONL thickness
