Passive Cutaneous Anaphylaxis Protocol

Local cutaneous anaphylaxis is a type I hypersensitivity reaction mediated by the cross-linking of antigens to immunoglobulin E (IgE) on the surface of mast cells and basophils. Quantification of these allergic responses can be achieved by using Evans Blue dye to measure changes in vascular permeability after antigen challenge. In passive cutaneous anaphylaxis (PCA), an exogenous IgE antibody specific against an antigen (e.g., 2,4-dinitrophenol) is injected intradermally in the ear. Several hours later the antigen is injected intravenously along with Evans blue dye to measure plasma extravasation.

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  • PCA MODEL INDUCTION
    photos of control antibody injected ear and DNP antibody injected ear in passive cutaneous anaphylaxis animal model

    The photo on the left is the control site, and the photo on right is the test site of induction. Challenge occurs 24-hr after induction. Photos were taken 40 minutes post challenge, and study-dependent endpoints are assessed as required.

  • STUDY ENDPOINTS
    • PK/PD blood collections
    • Ear thickness measurements
    • Cytokine/chemokine analysis
    • Clinical chemistry
    • Histopathological evaluation
    • Immunohistochemistry