Overview

  • IgE antibody-mediated basophil activation using flow cytometry in whole blood
  • Species: Rat

IgE-mediated basophil activation is a method to measure the potency of a molecule to prevent the development of allergic symptoms. In this assay, anti-IgE is used to stimulate IgE-mediated basophil activation by cross-linking anti-IgE receptors that trigger downstream signaling pathways. Following activation, basophils degranulate and express CD63 on the cell surface, whereas resting basophils have intact intracellular granules and do not express CD63 on the cell surface. Basophil degranulation is then analyzed by flow cytometry using a multi-color panel that identifies the basophil population (High-Affinity-Receptor (HAR)-positive cells) and the percentage of activated basophiles (CD63-positive cells) in rat whole blood.

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graph showing the expression of CD63 on the cell surface

Figure 1: Ex vivo basophil activation assay – Following the administration of anti-IgE antibodies, basophils are shortly activated as evidenced by the expression of CD63 on the cell surface. The administration of an inhibitor of basophil degranulation prevents the exposure of CD63 on the cell surface.