Bioanalytical Services

Accuracy and sensitivity are critical parameters for bioanalysis, and Charles River provides a wide range of bioanalytical services. A combination of expert scientists and state-of-the-art equipment allows us to offer novel analytical techniques, including SymDaq, our proprietary derivatizing agent, to support analysis of low concentrations of multiple neurotransmitters simultaneously in a single run. Additionally, we can develop novel study designs based on your specific needs for sample preparation, quantification, and analysis of samples.

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Figure 1: SymDaq – Multiple Neurotransmitters in a Single Run

chart showing the measurement of multiple neurotransmitters using LC-MS-MS

SymDAQ enables simultaneous analysis of multiple neurotransmitters (NE, DA, 5HT, HA, GABA, Glycine, and Glu) in a single dialysis sample.
 

CASE STUDY: MEASURING MULTIPLE NEUROTRANSMITTERS

  • Situation

    Client needed to identify a pharmacodynamic measurement for their compound, but didn’t know where to start.

  • Approach

    A program was designed to leverage our SymDaq extraction method for increased accuracy and sensitivity in LC-MS/MS detection, as well as lower sample volumes, and enhanced overall sensitivity and efficiency.

  • Result

    We were able to identify specific changes in low levels of one of the 18 transmitters measured, allowing our client to better understand the mechanistic changes evoked by their compounds. This data aided in compound selection, as well as further clinical development.

Customized Solutions

Additionally, we can develop novel study designs based on your specific needs for sample preparation, quantification, and analysis of samples.

  • Biological Matrices
    • CSF samples
    • Microdialysates
    • Brain tissue samples
    • Plasma, plasma free fraction or serum
    • In vitro matrices
    • Urine
    • Bone
    • Gut tissue
    • Liver
    • Kidney
    • Spleen
    • Human tissue (non-GLP)
    • Other biological tissues
    • Other biological fluids
  • Existing Analyses
    • Acetylcholine
    • Amino acids (including GABA)
    • cAMP/cGMP
    • Glucose and lactate
    • Histamine
    • Monoamines (including NE, DA, and 5HT)
    • Exogenous compounds
    • Glucocorticoids
    • Kynurenines
    • Tryptophan
    • MA metabolites (including HVA, 5-HIAA, and DOPAC)
    • D- and L-Serine
    • Polyamines (including putricine and spermine)
    • Custom analyte services