Neurochemistry: Bioanalysis Services

Accuracy and sensitivity are critical parameters for neurotransmitter detection. Our state-of-the-art equipment allows us to offer novel analytical techniques, including SymDaq, our proprietary derivatizing agent, to support analysis of low concentrations of multiple neurotransmitters simultaneously in a single run. Additionally, we can develop novel study designs based on your specific needs for sample preparation, quantification, and analysis.

Sym DAQ LC-MS enables simultaneous analyses of multiple neurotransmitters in a single dialysis sample

An LC-MS plot of multiple neurotransmitters in a single dialysate sample by the Charles RIver proprietary SymDAQ technology.


Does Ketamine Really Modulate Glutamate?

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Neurochemistry Tools and Sample Types:

  • Neurochemistry Bioanalytical Tools:
    • HPLC – tandem mass spectrometry
    • HPLC – electrochemical detection
    • HPLC – fluorescence detection
    • RIA / ELISA
  • Biological Matrices:
    • CSF samples
    • Microdialysates
    • Brain tissue samples
    • Plasma, plasma free fraction or serum
    • In vitro matrices
    • Urine
    • Bone
    • Gut tissue
    • Liver
    • Kidney
    • Spleen
    • Human tissue (non-GLP)
    • Other biological tissues
    • Other biological fluids
  • Neurochemistry Analytes:
    • Acetylcholine
    • Amino acids (including GABA)
    • cAMP/cGMP
    • Glucose and lactate
    • Histamine
    • Monoamines (including NE, DA, and 5HT)
    • Exogenous compounds
    • Glucocorticoids
    • Kynurenines
    • Tryptophan
    • MA metabolites (including HVA, 5-HIAA, and DOPAC)
    • D- and L-Serine
    • Polyamines (including putricine and spermine)
    • Custom analyte service

Electrochemistry: Fast-Scan Cyclic Voltammetry

Beyond traditional electrophysiology studies, Charles River provides a unique electrochemistry platform called fast-scan cyclic voltammetry (FSCV). FSCV is used to monitor the release of dopamine following electrical stimulation in the brains of animal models. It is a great solution when you need to track very rapid changes in dopamine concentrations. FSCV utilizes a carbon fiber microelectrode to apply a voltage which results in current changes of the surrounding environment.

FSCV electrochemistry color plot and evoked phasic dopamine release in a mouse brain

An electrochemistry experiment showing a color plot of the voltammetry changes from electrically-evoked dopamine release in a mouse brain. A chart on the right showing in vivo recording of phasic dopamine release in rodents upon increasing electrical stimulation.
Dopamine concentration increase correlates to electrical stimulation intensity in the graph above. Raclopride, a dopamine receptor antagonist, decreases overall dopamine release.

FSCV works by applying a triangular voltage wave form at the site of dopamine release. Changes in the current are recorded simultaneously (10 times/second), specifically focusing on the oxidation of dopamine to dopamine-O-quinone and its reduction back to dopamine. The resulting current measurements can then be used to quantify the instantaneous concentration of dopamine in the extracellular space. This electrochemistry technique enables quantification of dopamine changes in animal models in real-time, adding further value when combined with other services and techniques as part of a customized program of study.


Comprehensive Bioanalysis is Key at Every Stage of Product Development

Bioanalytical services include careful preparation of reference standards for a validated
bioanalytical method standard curve to quantitate biological samples for drug concentrations.

Bioanalysis is intertwined with our:

  • Discovery in vitro, in vivo and ex vivo studies
  • Preclinical research
  • Clinical capabilities

Learn about our other Bioanalysis methods and tools



Frequently Asked Questions (FAQs) for Neurochemistry & Electrochemistry

Have more questions on electrochemistry and neurochemical bioanalysis for your studies?

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