Endotoxin-Induced Anterior Uveitis in Rabbits
For this assay, E. coli-derived LPS is injected into the vitreous humor of New Zealand White rabbits. This produces an acute transient inflammatory response that resolves spontaneously within approximately 72 hours. During this time period, the eyes can be evaluated by slit lamp ophthalmological examination. Aqueous humor can be tapped and evaluated for the amount of inflammatory cells present and the level of cytokines. Additionally, anti-inflammatory drug candidates can be screened against vehicle formulations to determine relative efficacy.
Study Endpoints
- Slit lamp ophthalmology examination
- Aqueous humor (AH) cell counts
- All endpoints completed by 72 hours post LPS
| Treatment | AH Cell Count Average | AH Cell Count Range |
|---|---|---|
| None | 1.0 cells/uL | 0 - 4 cells/uL |
| LPS + Topical Saline | 71 cells/uL | 5 - 390 cells/uL |
| LPS + Topical Corticosteroid | 31.5 cells/uL | 2 - 57 cells/uL |