Angiogenesis plays a major role in supplying nutrients and oxygen in embryogenesis, wound healing, and placental implantation for the delivery of nutrients and the removal of waste products. In pathologic conditions, such as tumor growth, diabetic retinopathy, psoriasis, and rheumatoid arthritis, the angiogenic response plays a role in disease progression. As a result, angiogenesis has become a prime target for novel therapies in a variety of medical indications.
The study and development of anti-angiogenic therapies depends on reliable and reproducible stimulation models of neovascular response. Charles River has several experimental techniques as part of our angiogenesis cancer model that allow an effective examination of short-term neovascularization and angiogenic response to bFGF, VEGF or other angiogenic stimuli:
Corneal Micropocket Assay
- Hydron pellet prepared with growth factors chosen for specific study requirements
- Surgical implantation of pellet into a corneal micropocket
- Corneal neovascularization measured using slit-lamp microscope
- Short-term study with rapid turnaround times
In Vivo Screens Using Angiogenic-Dependent Tumor Growth
- Bevacizumab can be used as a positive control
- Long-term, low-level dosing of cytotoxics or other agents of interest to inhibit growth and development of vascular endothelium while avoiding whole-animal toxicity
- Metronomic dose determination for use in efficacy study
- Studies can last up to 90 days
1. Browder, 2000; Cancer Research 60:1878
Frequently Asked Questions (FAQs) for Angiogenesis Cancer Models
What is an angiogenesis cancer model?
Angiogenesis cancer models are animal models that are used to study tumor angiogenesis. Angiogenesis itself contributes to tumor growth by extending new blood vessels into the tumor and therefore supplying new nutrients and oxygen, aiding tumor growth. However, quantification of angiogenesis within the tumor can be problematic and subjective, so an ideal screening model for cancer angiogenesis is the corneal micropocket assay. This is a fully quantifiable model and therefore allows efficient screening of anti-angiogentic compounds and can be used alongside tumor models.
How can the corneal micropocket assay be used to study cancer angiogenesis?
Traditional tumor angiogenesis models can be difficult to quantify, so an ideal screening model for cancer angiogenesis is the corneal micropocket assay. This angiogenesis cancer model is a robust and fully quantifiable, as it uses accurate measurement of corneal neovascularization to mimic the angiogenesis process seen in tumors.