In vivo Optical Imaging: Bioluminescence and Fluorescence
Optical imaging (bioluminescence and fluorescence) can be applied to preclinical models to help visualize a number of possible biological events happening within a live animal. This technique can help monitor tumor growth and metastasis as well as see where and when various molecular processes are taking place.
Our team has validated the use of 2D and 3D in vivo optical imaging for applications such as:
- Biodistribution of antibodies
- Distribution of immune cells in tumor-bearing animals
- Quantification of cancer-related biomarkers
- Size and location of tumors
Imaging data can help researchers optimize dose and delivery strategies, which ultimately improves a therapy’s translation to the clinic. More complex studies use a combination of luciferase and fluorescence imaging to track two differently radiolabeled tumors and therapies within a precise anatomical microenvironment to reveal new options to improve treatment outcomes.
Why is imaging important in preclinical drug development?
- Is a fast, sensitive and reliable method to determine tumor load
- Helps assess anatomy and disease morphology in a very exact manner
- Follows metastatic progression after orthotopic implant
- Reduces the total number of mice per study with follow up of each animal at several time points (longitudinal study – clinically relevant setting)
- Is suitable for a wide variety of in vivo and in vitro models, including: