hACE2-NCG Mouse Details
The Charles River hACE2-NCG mouse is a novel, engineered hACE2-modified mouse developed specifically for COVID-19 research. The hACE2-NCG model is developed on the NCG triple-immunodeficient background containing hACE2 (Angiotensin Converting Enzyme 2), the receptor used by SARS-CoV-2 to enter cells.
The hACE2-NCG modified mouse model uses an optimized genetic modification strategy that knocks in human ACE2 at the mouse ACE2 locus. This introduces the hACE2 gene into the designated position on the NCG background, to increase susceptibility to SARS-CoV-2 infection. In this model, the intracellular domain of the mouse contains ACE2 under the transcriptional regulation of endogenous sequences, designed to mimic the physiological expression pattern of ACE2 in various tissue types, including the kidney, lungs, and intestines.
hACE2-NCG is Humanizable
The hACE2-NCG mouse was designed to support critical translational research intended to mimic various human immune responses following exposure to SARS-CoV-2. Through the engraftment of human cells, part of the immune system can be reconstituted prior to SARS-CoV-2 infection. The advantage of studying SARS-CoV-2 infections through human reconstitution could allow for the development of models used to study the mechanisms of different immune cells during infection.
NCG mice are available in North America only.
See our isolator page for immunocompromised animal housing units.
**CRISPR/CAS9 used under licenses to granted and pending US and international patents from The Broad Institute and ERS Genomics Limited.
*Pricing is subject to our General Terms & Conditions of Sale.
Frequently Asked Questions (FAQs) about the hACE2-NCG Mouse Model
How was the hACE2-NCG mouse model created?
The hACE-2-NCG mouse was developed using an optimized genetic modification strategy to introduce the hACE-2 gene into the designated position on an NCG triple immunodeficient background.
What types of studies can hACE2-NCG mice be used in?
The Charles River hACE2-NCG mouse is a novel, humanized, and humanizable hACE2-modified mouse developed specifically for COVID-19 and infectious disease research.
How is the hACE2-NCG model different from other COVID models?
The challenge has been that no animal model systems exist to study human immune responses in SARS-CoV-2 viral infections. The humanizable hACE-2 modified mouse, is the first model developed for SARS-CoV-2 and infectious disease research on an NCG triple immunodeficient background to assess human immune responses to SARS-CoV-2 infection.
Are NCG mice suitable for the creation of humanized mouse models?
The NCG mouse model is the foundation of our humanized mouse model portfolio. NCG mice have a unique phenotype that allows it to host xenograft cells, tissue, and components of the human immune systems. It displays noticeable improvements of foreign tissue transplantation and engraftment, compared to previous generations of immunocompromised mice.
In addition to offering the hACE2-NCG mouse and other humanized models, Charles River offers the NCG/PBMC Select Humanization Kit for you to create your own humanized mice model on your own timeline.