HuCD34-NCG Mouse Details
NCG mice are humanized by adoptive transfer of human umbilical cord blood-derived CD34+ stem cells from a qualified source, following myeloablation treatment. Animals are subsequently housed for 12-15 weeks according to Charles River immunodeficient animal housing protocols while the human CD34+ stem cells stably engraft within the immunodeficient NCG mice.
Human immune cells can be detected in the peripheral blood as early as 12-15 weeks post-engraftment. These immune cells can also be detected in mouse tissues, including bone marrow, spleen, liver, and lung at later time points.
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This model is available in North America only.
The HuCD34-NCG are an ideal in vivo platform to evaluate the effectiveness of compounds that function by modulating the human immune system. The lack of, or late onset of, graft-versus-host disease (GvHD) in these engrafted animals makes them ideal for longer term studies.
For more information on this model, download the HuCD34-NCG Mouse product sheet.
NCG Mouse Origin
The NCG mouse was co-developed by Nanjing Biomedical Research Institute of Nanjing University and Nanjing Galaxy Biopharma in 2014, and transferred to Charles River in 2016. This model was created by sequential CRISPR/Cas9 editing of the Prkdc and Il2rg loci in the NOD/Nju mouse, generating a mouse coisogenic to the NOD/Nju.
The NOD/Nju carries a mutation in the Sirpa (SIRP α) gene that allows for engrafting of foreign hematopoietic stem cells. The Prkdc knockout generates a SCID-like phenotype lacking proper T cell and B cell formation. The knockout of the Il2rg gene further exacerbates the SCID-like phenotype while additionally resulting in a decrease of NK cell production.
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- PRODUCT SHEET
- Evaluation of in vivo anti-tumor response of solid tumors in a novel immune cell humanized NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl mouse model
- The single mouse trial format predicts the sensitivity towards checkpoint
- NCG/CRL as a novel (Charles River) humanized mouse model for pre-clinical oncology studies: immunophenotypic characterization and performance monitoring