HuCD34-NCG Mouse

NOD-Prkdc26emCd52Il2rgem26Cd22/NjuCrl
Coisogenic
Immunodeficient
HuCD34-NCG Mouse
Breeding Location

HuCD34-NCG Mouse Details

NCG mice are humanized by adoptive transfer of human umbilical cord blood-derived CD34+ stem cells from a qualified source, following myeloablation treatment. Animals are subsequently housed for 12-15 weeks according to Charles River immunodeficient animal housing protocols while the human CD34+ stem cells stably engraft within the immunodeficient NCG mice.

Human immune cells can be detected in the peripheral blood as early as 12-15 weeks post-engraftment. These immune cells can also be detected in mouse tissues, including bone marrow, spleen, liver, and lung at later time points.


 
white mouse among test tubes


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This model is available in North America only.

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The HuCD34-NCG are an ideal in vivo platform to evaluate the effectiveness of compounds that function by modulating the human immune system. The lack of, or late onset of, graft-versus-host disease (GvHD) in these engrafted animals makes them ideal for longer term studies.

For more information on this model, download the HuCD34-NCG Mouse product sheet.

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NCG Mouse Origin

The NCG mouse was co-developed by Nanjing Biomedical Research Institute of Nanjing University and Nanjing Galaxy Biopharma in 2014, and transferred to Charles River in 2016. This model was created by sequential CRISPR/Cas9 editing of the Prkdc and Il2rg loci in the NOD/Nju mouse, generating a mouse coisogenic to the NOD/Nju.

The NOD/Nju carries a mutation in the Sirpa (SIRP α) gene that allows for engrafting of foreign hematopoietic stem cells. The Prkdc knockout generates a SCID-like phenotype lacking proper T cell and B cell formation. The knockout of the Il2rg gene further exacerbates the SCID-like phenotype while additionally resulting in a decrease of NK cell production.

Coat Color
White (albino)

Strain Code
695

Ideal For
Oncology, immunology, hematopoiesis, stem cell research, infectious disease, regenerative medicine, transplantation research, hematological disease

Not available.

LOCATION: Wilmington
UNIT: W8H/W08
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