HuPBMC-NCG Mouse Details
In vivo models engrafted with human immune cells are critical for immuno-oncology, infectious disease, and graft rejection research. Peripheral blood mononuclear cells (PBMCs) are of particular interest when humanizing mice because of their ability to quickly engraft in immunodeficient mice.
Combined with our triple-immunodeficient NCG mouse model (strain code 572; lacking functional/mature T, B, and NK cells, along with reduced macrophage and dendritic cell function), pre-qualified PBMCs allows researchers to quickly confirm results, without the need to qualify donor cells. This humanized PBMC (hu-PBMC) mouse model is ideal for short-term studies requiring a strong effector and memory T-cell and NK-cell function.
The study-ready HuPBMC-NCG model offers the benefits of convenience, efficiency, as well as excellent model quality through trusted and validated PBMC source. Moreover, we also offer the flexibility of DIY PBMC kit, providing researchers the flexibility of engrafting on a timeline of their choice. The majority of mature human immune cells, when using PBMCs, are of human T cell origin (CD4+ and CD8+) within a week to 10 days.
In addition to offering the HuCD34-NCG mouse and other humanized models, Charles River offers the NCG/PBMC Select Humanization Kit for you to create your own humanized mice models on your own timeline.
NCG Mouse Origin
The NCG mouse was co-developed by Nanjing Biomedical Research Institute of Nanjing University and Nanjing Galaxy Biopharma in 2014, and transferred to Charles River in 2016. This model was created by sequential CRISPR/Cas9 editing of the Prkdc and Il2rg loci in the NOD/Nju mouse, generating a mouse coisogenic to the NOD/Nju.
The NOD/Nju carries a mutation in the Sirpa (SIRP α) gene that allows for engrafting of foreign hematopoietic stem cells. The Prkdc knockout generates a SCID-like phenotype lacking proper T cell and B cell formation. The knockout of the Il2rg gene further exacerbates the SCID-like phenotype while additionally resulting in a decrease of NK cell production.
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