Immortomouse

CBA;B10-Tg(H2Kb-tsA58)6Kio/Crl
Genetically Engineered

Immortomouse Details

At the Ludwig Institute for Cancer Research, a hybrid construct containing H-2Kb(MHC Class I antigen) 5' promotor sequences fused to the early region of the SV40 mutant tsA58, which encodes both the large and small SV40 tumor antigens, was microinjected into fertilized oocytes from CBA/Ca x C57BL/10 F1 mice. Following reimplantation, 88 mice were born, of which 34 were transgenic and carried one to five copies of the gene. RNA from a variety of tissues from one nontransgenic and three transgenic animals was analyzed by Northern blot analysis using a SV40 early region-specific probe. RNA extracted from tissues of transgenic mice contained varying amounts of a 2.5kb RNA species, while no tsA58TAg RNA was detected in tissues of the nontransgenic mouse; thymus and liver showed the highest level of expression, while brain showed the lowest. Distribution rights to Charles River in 1991.

Coat Color
Primarily agouti, infrequently black

Strain Code
This model is cryopreserved.

Ideal For
Oncology

Bred in

The Immortomouse® model is cryopreserved. For more information, contact us at [email protected].

The Immortomouse® model is cryopreserved. For more information, contact us at [email protected].

The Immortomouse® model is cryopreserved. For more information, contact us at [email protected].