HT-ADME Assays
With decades of experience running in vitro ADME assays and analyzing the data, we can offer those same in vitro assays and bioanalyses more efficiently to generate ADME data earlier in the drug discovery process. This high-speed process optimization and reproducibility can generate quality ADME data for every compound in your chemical library.
To help streamline the early drug-discovery process, we have successfully validated and deployed automation and IT infrastructure to enable rapid delivery of in vitro ADME results. The workflow enables up to 500 compounds per week to support primary assays:
- Microsomal stability
- Permeabililty (Caco-2 and MDCK-MDR1)
- Protein binding
- RBC partitioning (Blood/Plasma ratio)
High-Speed Data Acquisition
Figure 1: 160 permeability samples analyzed in 30 minutes
Validation Data
Below are manual vs. HT LC-MS/MS and automated liquid handling workflows using new chemical entities (NCEs) and marketed compounds.
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MICROSOMAL STABILITY ASSAY
Figure 2: Microsomal – HT LC-MS automated vs. manual
Figure 3: Microsomal stability – HT assay performance across multiple runs
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CACO-2 PERMEABILITY ASSAY
Figure 4: Consistent automated assay performance
Figure 5: Similar performance UPLC-MS vs. HT-MS (ADDA)
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PROTEIN BINDING ASSAY
Figure 6: Protein binding – HT LC-MS automated vs. manual
Figure 7: Protein binding – consistent automated assay performance over time
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RBC PARTITIONING ASSAY
Figure 8: RBC partitioning – HT LC-MS vs. UPLCMS