Immunosuppressive/Immunostimulatory Potential Evaluation

Our immunology groups can evaluate a compound’s potential to be an immunosuppressor or an immunostimulator. We can provide you with fully integrated services to support toxicology and clinical studies that determine the safety of compounds and define immunological endpoints and alterations of cellular function induced by test compounds.

Assays for Measuring Humoral & Cellular Immune Responses

  • T cell-dependent antibody response – TDAR (ELISA) with various model antigens, including for immune stimulation assessment
  • Extended histopathological examination: lymphoid tissues and organs
  • Immunophenotyping in blood or organs (flow cytometry)
  • Neutrophil/Macrophage (flow cytometry)
    • Oxidative burst activity
    • Phagocytosis
    • Migration
  • Complement (ELISA, flow cytometry)
  • Cytokine/Chemokine (ELISA, flow cytometry)
    • Profiling
    • Release
  • Cell proliferation (beta counter, flow cytometry)
  • Natural killer (NK) cell activity (gamma counter, flow cytometry)
  • Basophil (flow cytometry)
  • T-cell cytotoxicity
  • ADCC/CDC (flow cytometry)
  • ELISpot and FluoroSpot
  • Extracellular markers of cell activation, indicators of cell injury and death, and receptor occupancy and test article binding (flow cytometry)
  • Immunohistochemistry and tissue cross-reactivity
  • Anti-dsDNA and antinuclear antibodies
  • Screening for autoantibodies and autoimmune reactions