Gel-Clot Endotoxin Test Reagents & Endpoint Chromogenic LAL Kit
The gel-clot assay determines the presence of endotoxins through its clotting response, and is a quick and effective way to detect harmful endotoxins for your injectable pharmaceuticals. Endosafe® Rapid LAL Single-Test Vials contain FDA-licensed LAL to provide a simple, self-contained option for routine endotoxin detection in research samples, renal dialysis process water, and dialysate solutions.
Gel-Clot LAL Reagents Product Codes
Product Sensitivity EU/mL Code 50-test vial (5.2 mL) 0.015 R15015 0.03 R15003 0.06 R15006 0.125 R11012 0.25 R11025 10-test vial (1.2 mL) 0.03 R12003 0.06 R12006 0.125 R12012 0.25 R12025
Endpoint Chromogenic LAL Kit Product Codes
Product Sensitivity EU/mL Code Gel-Clot LAL Single-Test Vial (0.2 mL) 0.06
0.06 or 0.25
Rapid single-test vial (50-test) 0.06 or 0.25 R13500 Rapid single-test vial (50-test) 0.25 or 1.0 R13600 Rapid positive product control (50-test) n/a PC200
Endpoint Chromogenic Reagent Product Code
Our gel-clot endotoxin test endpoint chromogenic reagent is a non-kinetic LAL assay that yields quantitative results quickly over a narrow standard curve range. This gel-clot endotoxin test reagent eliminates the need for an incubating plate reader, decreasing the testing time in comparison to a standard gel-clot endotoxin test. For many applications, the Endosafe® nexgen-PTS™ offers advantages of speed and convenience to the endpoint user.
Product Code Endpoint Chromogenic Kit - 140 tests R160
Frequently Asked Questions (FAQs) About Gel-Clot Endotoxin Testing
How does the gel-clot endotoxin test work?
The gel-clot endotoxin test method will show the presence of endotoxins based on whether gel clot forms in the sample tube when introduced to the LAL reagent. If endotoxin is present in the sample, a gel clot will occur when coming in contact with the LAL reagent. If the clot stays at the bottom of the test tube after inverting it, the sample is positive for endotoxin.
When would you use a gel-clot endotoxin test?
A gel-clot endotoxin test is one of the more simple assays that can be conducted that yields qualitative results indicating the presence of endotoxins. As the original BET method of testing, the gel-clot endotoxin test should be used when you have samples that:
- Are extremely viscous
- Are extremely colorful such as red, orange, and yellow
- Contain particles, precipitation, or Trypsin
- Can cleave pNA
If you are testing more than 10 samples a month, prefer endotoxin calculations to be performed using validated software, or quantitative results over qualitative results, we recommend moving to a kinetic turbidimetric endotoxin testing method or using our Endosafe® cartridge technology with one of our endotoxin testing instruments: the Endosafe® nexgen-PTS™, the Endosafe® nexgen-MCS™ or the Endosafe® Nexus™.
What equipment is used when performing a gel-clot endotoxin test?
The gel-clot endotoxin test uses a heater block or water bath for instrumentation and several accessories, such as dilution tubes, pipettes, and pipette tips.
Why would I switch from a gel-clot endotoxin testing method to a Charles River Endosafe® cartridge technology?
There are several reasons to switch to Endosafe® cartridge technology from the gel-clot method that would benefit your company, such as:
- Reduce chance for human error
- Increase data integrity
- Reduce LAL usage
- Purchase less accessories
- Reduce retest rates
- Reduce technician training time
- Where do I find verification that the gel-clot endotoxin test is a compendial method?