Robust Reagents. Rapid Results.

Traditional methods of microbial testing rely heavily on lengthy incubation periods to achieve visible growth, for colonies to grow large enough for confident enumeration in microbial limits, or for unambiguous turbidity in sterility testing. Celsis® ATP bioluminescence-based rapid detection can reduce these incubation periods by 50%.

As an automated rapid microbial test for aseptic and sterile manufacturing processes, Celsis® ATP-bioluminescence reagents will quickly screen for the absence or presence of bacteria, yeasts, and molds, allowing the release of final products through instrument-based analysis, following a shortened incubation time.

Celsis® ATP-bioluminescence Reaction

firefly with bioluminescence tail

The fundamentals of Celsis® reagents are simple, yet powerful. Celsis® rapid microbial detection reagent technology is based upon a naturally occurring, ATP-bioluminescence reaction, which also provides the characteristic glow of fireflies.

What is the ATP-bioluminescence Assay?

Commonly known as the luciferin-luciferase reaction or luciferase assay, this reaction occurs when the luciferase enzyme converts luciferin into oxyluciferin to produce light in the presence of adenosine triphosphate (ATP). Only when ATP is present will this reaction occur.

Known as the luciferin-luciferase reaction, it occurs when the luciferase enzyme converts luciferin into oxyluciferin to produce light. Only when ATP is present will this reaction occur. Since ATP is present in all living cells, including prokaryotes, it can be used as a microbial testing marker for contamination if a sample should not, under normal conditions, contain ATP.

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Celsis AMPiScreen Amplified bioluminescence

Celsis AMPiScreen® reagents rapidly detect the presence of ultra-low levels of microbiological contamination up to 50% faster than other on-market ATP bioluminescence methods, with compatible sample types. The amplified-ATP assay, used in Celsis AMPiScreen®, is not constrained by the limited amount of metabolic ATP in the organism. Instead, it produces additional ATP by using an enzyme-catalyzed reaction and an additional instrument-controlled amplification period to optimize the detection of microorganisms. Learn More

Features of Celsis® Rapid Microbial Detection Reagent

  • Celsis AMPiScreen® Reagent is compatible with Celsis Accel® and Celsis Advance II™ luminometers.
  • Optimized reagent kit size and volume to minimize waste and improve reagent consumption efficiency.
  • Leverages the same in-house expertise that produces our industry-leading FDA-licensed Endosafe® reagents, trusted across the pharmaceutical industry.

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Frequently Asked Questions (FAQs) About ATP-Bioluminescence Reagents

  • What is the ATP-bioluminescence assay used for?

    The ATP bioluminescence can be used as a screening assay for the presence of adenosine triphosphate (ATP) present in a sample when no ATP is present. Since ATP is present in all living cells (including those of bacteria, yeasts, and molds), it can be used in microbial testing to detect the presence of contamination in a sample.

  • Why is ATP-bioluminescence a gold standard detection method?

    ATP-bioluminescence, utilizing the luciferase assay, is considered a gold standard detection due to its highly efficient ability to detect microbial contamination at extremely small concentrations. When this reaction is controlled and allowed to exponentially react using the timed injection of specialized reagents, the reaction provides positive confirmation of contamination.

  • How long has ATP-bioluminescence been used for testing?

    ATP-bioluminescence, with numerous scientific publications studying its properties, has been determined a means for microbial detection since 1989. The ATP-bioluminescence assay used in Celsis AMPiScreen® reagents was first characterized in 1955, when it was known as the adenylate kinase assay at the time. It has been described in microbial testing since 1998.