It’s all about the validated library and reporting process when you choose to sequence your microorganism (BacSeq or FunITS) and analyze the data with an optimized, manual cGMP-compliant process. Once the DNA sequence is analyzed, it is compared to a relevant, validated library of known organisms, and an identification report is generated and interpreted.
Interpretation of the sequence, library comparison and report generation are critical parts of the process. There are no interpretation rules which can be applied universally. The species cutoff varies from genus to genus. Sometimes the species that comprise a particular genus are very closely related (i.e., low genetic distance), but in other genera the species are only distantly related (i.e., high genetic distance). This variation in distance measurements makes species interpretation using only one genetic distance cutoff impossible. One must first look at the genetic distance of the unknown to its closest match and then determine if that distance is on average equal to or less than the distance that separates the known species of that particular genus. Thus, the education and experience of our Microbial Phylogeneticists are crucial to assigning the correct identification.