Bacterial ID with AccuGENX-ID® BacSeq
Accurate identification of bacterial isolates is critical to environmental monitoring programs. Genotypic methods, such as comparative sequencing of the 16S ribosomal RNA (rRNA) gene in bacteria, have been proven to be the most accurate and reproducible methods for identifying unknown organisms. The technology is inherently stable, which allows for reproducible data for identification.
For many organisms, traditional phenotypic identification is problematic, takes a great amount of time and can result in subjective interpretation of the test results. Not all strains within a given species consistently exhibit a particular characteristic, thereby limiting phenotypic identification methods. Additionally, library databases utilized in support of phenotypic identification are limited.
Our AccuGENX-ID® BacSeq genotypic analysis process adheres to the reference method used by taxonomists because it delivers the most accurate and reliable sequence data for identifications. When this process is combined with the full coverage, validated Accugenix® library, the resulting phylogenic analysis is unparalleled in the industry.
Protein-Coding Gene Sequencing with AccuGENX-ID® ProSeq
Identification with ProSeq is a highly discriminatory and accurate sequence-based method that can be used to distinguish between very closely related microorganisms to the species level. Resolution is achieved by analyzing protein-coding genes.
If the AccuGENX-ID® report contains a species* confidence level (indicating the identification resolution is to the group or complex level), and we have determined the target gene that can distinguish between the different species in that group of organisms, customers can request our identification with ProSeq service.
Eligible species include Burkholderia cepacia complex (BCC) among others. Please contact us for a full list of available closely related microorganism groups for species-level identification.