Transgene Copy Number
Transgenes typically insert randomly in the genome as tandem repeats, but the site of insertion and number of copies varies in each founder line. Using qPCR, we determine relative copy number for each founder line, providing valuable information such as transgene instability and segregation of closely linked insertions, both commonly overlooked factors influencing the consistent phenotype of a colony. Undetected alterations may be passed through the germline, making copy number determination an important quality control tool for your transgenic models.
The relative or absolute transgene copy number may also help explain changes of phenotype or inconsistent performance of the colony, both crucial for studies such as disease phenotype and preclinical drug efficacy analysis.