It’s often difficult to discriminate between homozygous and hemizygous transgenic animals using traditional PCR, but the distinction between these zygosities is critical to transgenic breeding strategies, as well as planning study cohorts.
We’ll provide a definitive report on the zygosity of your animals quickly and accurately using our high-throughput, state-of-the-art qPCR technology.
- Measurement of fold difference from known heterozygous control
- Two known heterozygous controls used for calculations
- Both target and endogenous control are tested in quadruplicate
- Analysis is performed using delta-delta CT – relative quantitation method