Overview

We offer comprehensive testing capabilities to ensure that your animals are germ free. Our germ-free testing service is designed for evaluation of germ-free environments using aerobic and anaerobic culture of samples obtained from the isolator environment, individual cages, and animals. We also offer next-generation sequencing (NGS) to measure diversity of bacterial species in colony animals. In addition to our diagnostic services, we offer a portfolio of products and services to directly support your microbiome research.

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C57BL/6 Germ-Free Mouse is Now Available for Your Research
C57BL/6-Germ-Free Mouse

Bred in an axenic environment to ensure strict exclusion of all microorganisms, this mouse is an indispensable model for research into the host-microbiome interaction, including type 1 diabetes, inflammatory bowel disease, obesity, and autism. Learn More

Germ-Free Testing

Our microbial cultures can confirm the absence of bacteria in samples from germ-free animals. If bacterial growth is detected, we will work to identify the bacteria, and assist in preventing the same agent from showing up in the future.

  • Aerobic Culture: Plated onto both general and specialized media to help recover even organisms that are less hardy, then incubated promptly after receipt in order to ensure organism viability is preserved.
  • Anaerobic Culture: Plated on culture media under anaerobic conditions (anaerobic chamber), then a wet mount is performed for observation of bacterial cells.
  • qPCR Testing for Viral Agents
  • Microbial ID: In the unfortunate event that culture growth is detected in your sample colony, identification is available.

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Next-Generation Sequencing

NGS allows researchers to measure the diversity of bacterial species within a sample. This is essential when trying to identify a link between disease and microbiome composition.

Using the latest technology, our laboratory will extract genetic material from submitted fecal samples, run analysis to determine the presence and quality of 16S Ribosomal RNA, and use NGS technology to produce 16S sequence for downstream analysis.

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