We offer comprehensive testing capabilities to ensure that your animals are germ free. Our germ-free testing service is designed for evaluation of germ-free environments using aerobic and anaerobic culture of samples obtained from the isolator environment, individual cages, and animals. We also offer next-generation sequencing (NGS) to measure diversity of bacterial species in colony animals.
Our microbial cultures can confirm the absence of bacteria in samples from germ-free animals. If bacterial growth is detected, we will work to identify the bacteria, and assist in preventing the same agent from showing up in the future.
- Aerobic Culture: Plated onto both general and specialized media to help recover even organisms that are less hardy, then incubated promptly after receipt in order to ensure organism viability is preserved.
- Anaerobic Culture: Plated on culture media under anaerobic conditions (anaerobic chamber), then a wet mount is performed for observation of bacterial cells.
- qPCR Testing for Viral Agents
- Microbial ID: In the unfortunate event that culture growth is detected in your sample colony, identification is available.
NGS allows researchers to measure the diversity of bacterial species within a sample. This is essential when trying to identify a link between disease and microbiome composition.
Using the latest technology, our laboratory will extract genetic material from submitted fecal samples, run analysis to determine the presence and quality of 16S Ribosomal RNA, and use NGS technology to produce 16S sequence for downstream analysis.