NGS Microbiome Analysis

16S Next Generation Sequencing simultaneously sequences millions of short-product, double-stranded DNA templates. Using broadly reactive 16S PCR primers, we can determine the community composition of the microbes in your sample.


How 16S NGS Benefits Your Microbiome Research

  • Start-to-finish, non-GLP integrated service pipeline
  • Generates over 100,000 16S sequence reads per sample to ensure full microbial community representation
  • All samples are normalized and standardized
  • Robust and powerful bioinformatics tools, including secure private data storage and portal access through our partners
  • Convenient, complimentary shipping and collection supplies, and seamless submission of samples through LTM™
  • Multiple service options tailored to meet your research needs
Options
Microbiome Basic
Microbiome Plus
Advanced Analysis
What are You Looking For?
  • For Charles River to do DNA extraction, library prep, and sequencing while I do my own analysis
  • For Charles River to do DNA extraction, library prep, and sequencing
  • To get a standard report analysis with one-way comparison for finite sample number
  • For Charles River to perform DNA extraction, library prep, and sequencing
  • Advanced data analysis including microbial composition & diversity together with in-depth functional analysis
Analysis Partner
What You Get
  • Standardized report analysis with finite sample group with FASTQ access and data storage
  • Interactive, shareable, and archived results portal
  • Access to perform your own comparisons and analysis 
  • Data QC and normalization
  • Microbial composition and relative abundance per sample
  • Alpha and beta diversity analysis 
  • Interactive portal with sharable and archived data 
  • Create reproducible reports in other formats
  • PDF report that provides the relative abundances of alpha and beta diversity and Principle Component Analysis (PCA) plot 
  • Data QC and normalization, including Principle component and relative abundance per sample
  • Microbial composition and relative abundance per sample
  • Alpha and beta diversity analysis
  • Dynamic interaction with the Fios team as they work with your data to answer your research questions. Complete transparency regarding analysis methods used
  • Statistical comparisons between sample groups/conditions: differential abundance and functional profiling with in-depth network and pathway analysis
  • Possibility to integrate other types of data to the analysis:omics data or any other quantifiable data (e.g. behabior, cytokine, etc.)
  • Biological interpretation of the analysis outcomes
  • Analysis methods and outcomes delivered in a unique interactive, shareable, and archived portal. Analysis methods and outcomes can also be downloaded in a PDF report format

 

Applications for Next Generation Sequencing

  • Health Monitoring

    Next generation sequencing can be used for routine animal health monitoring, germ-free animal contaminations, confirmation of monocolonization or specific consortia colonization, and preserving microbiome stability over time.

  • Therapeutic Area

    Nearly all research studies are affected by the microbiome. The commensal microbiome plays a critical role or modifies severity of phenotypes in the following diseases/research areas, including oncology, metabolic disease, inflammatory bowel disease, cardiovascular disease, neurological disorders, and more.

vial and syringe

An Easier Way to Submit Your Samples

Our Laboratory Testing Management® (LTM™) platform centralizes your health, biomarker, and genetic testing programs into one virtual location so you can manage your samples easier.

Get Started