HuCD34-NCG Mouse Model
The HuCD34-NCG mouse from Charles River is a study-ready mouse model with a human-like immune system, created by adoptive transfer of CD34+ stem cells. HuCD34-NCG mice are an ideal in vivo platform to evaluate the effectiveness of compounds modulating the human immune system. The lack, or late onset, of graft-versus-host disease (GvHD) in humanized mice make them ideal for long-term studies.
NCG mice are humanized by adoptive transfer using human umbilical cord blood-derived CD34+ stem cells, following myeloablation treatment. Animals are subsequently housed for 12-15 weeks according to Charles River immunodeficient animal housing protocols.
During this period, CD34+ stem cells develop into human immune cells that engraft within the immunodeficient NCG mice. The presence of human immune cells are verified in the peripheral blood as early as 12-15 weeks post-engraftment, as verified by multi-color flow cytometry. These immune cells are detectable in mouse tissues, including bone marrow, spleen, liver, and lung, and have been shown to infiltrate implanted tumors.
The HuCD34-NCG mouse model is available in North America only.
Benefits of the HuCD34-NCG Mouse Model
- Convenient – HuCD34-NCG mice are study-ready, expressing human immune cells
- Consistent – Every mouse is pre-tested for humanization
- Stable – Engraftment of hematopoietic cells provides a research platform for extended studies
- License-free – No additional licensing fees or royalty payments
- Stem cell research
- Infectious disease
- Regenerative medicine
- Transplantation research
- Hematological disease
For more information on this model, download the HuCD34-NCG Mouse product sheet.
Pricing and Ordering Information
Frequently Asked Questions (FAQs)
At what age are the NCG mice injected with the human CD34+ stem cells?
Animals are injected at approximately five weeks of age (typically between 4-6 weeks of age).
What is the source of the CD34+ stem cells?
Human CD34+ stem cells are isolated from donor-consented, non-embryonic umbilical cord blood.
How long does it take for the CD34+ stem cells to develop into immune cells following injection of the cells?
Typically stem cells will immediately develop into immature and mature immune cells following injection. At approximately 12-14 weeks post injection, the majority (>30-50%) of human mature immune cells (hCD45+) are lymphocytes.
When can the HuCD34-NCG mice be placed on study?
Animals can be placed on study following standard acclimatization periods following shipment to your institute.
Why is the NCG mouse model ideal for CD34+ stem cell engraftments?
The NCG (NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl) is severely immunodeficient, lacking T, B, and active NK cells. The lack of immune mouse cells makes NCG an ideal host for immune cell humanization.
What is the protocol for CD34+ stem cell engraftment?
The protocol for stem cell engraftment begins with immune ablation using an X-ray irradiator. Following exposure, animals are injected with sufficient CD34+ cells purified from umbilical cord blood.
Will each HuCD34-NCG mouse be tested for engraftment?
How are the HuCD34-NCG mice tested for engraftment?
Blood samples from each animal will be analyzed by multicolor flow cytometry for engraftment prior to release for order or shipment.
Do the human immune cells stay in the periphery or are they found in tissues?
Human immune cells are found distributed to peripheral tissues including bone marrow, liver, lung, and spleen.
Do I need a license to use the HuCD34-NCG mice for my research?
Are there any special husbandry requirements for the HuCD34-NCG mice?
Animals should be housed and cared for according to immunodeficient mouse protocols.