Rat & Mouse Embryo & Sperm Rederivation Services

Charles River can remove adventitious organisms, such as viruses, bacteria, and parasites, from your mice and rats with our rederivation services. Your rederived animals will meet our VAF/Elite® health status and will be maintained in a biosecure isolator environment to protect the health of your colony. We have rederived over 10,000 unique strains of mice and rats with 100% removal of unwanted organisms.

Service Requirements Deliverables Estimated Timeline
Rapid Rederivation – Embryo and Sperm Mouse:
  • 2-5 males: < 6 months old
  • 10 females: 3-4 weeks old
Rat:
N/A
  • Minimum 2 visibly pregnant, embryo recipient females
  • VAF/Elite® mice
6 weeks
Embryo Rederivation with Health Report Mouse:
  • 2 males: < 6 months old
  • 10 females: 3-4 weeks old
Rat:
  • 4 males: < 6 months old
  • 10-15 females: 10-12 weeks old
  • Minimum 10 offspring with complete health report
  • Conventional colony held until project completion
  • For homozygous x homozygous strains
  • VAF/Elite® mice or rats
12-15 weeks
Sperm Rederivation with Health Report Mouse:
  • 2 males: < 6 months old
  • 10 females: 3-4 weeks old
Rat:
N/A
  • Minimum 10 offspring
  • Complete health report
  • For homozygous x homozygous strains
  • VAF/Elite® mice
12-15 weeks
Embryo Rederivation with Homozygous Expansion Breeding Minimum of 5 breeding pairs
  • Minimum 10 offspring with complete health report
  • Conventional colony held until project completion
  • For homozygous x homozygous strains
  • VAF/Elite® mice or rats
6-9 months

Discuss your project/Request a quote

Embryo Rederivation: Recommended for use with homozygous lines, lines with multiple genetic mutations, or rats. Your non-rederived live colony will be maintained until your deliverables are met.

Sperm Rederivation: This service is recommended for use with nonhomozygous, single mutation mouse lines.

Rapid Rederivation: Rapid rederivation services significantly reduce the time and cost associated with the rederivation procedure for mice and rats. We guarantee to return at least two visibly pregnant females to clients in as little as six weeks.

IVF Rapid Expansion: IVF-based rederivation and expansion for mice can provide a large quantity of animals to help obtain an established colony faster. Included in this service is a comprehensive health evaluation of the rederived colony to confirm the success of the expansion and guarantee that animals are of VAF/Elite® status.

Embryo Transfer: Embryo collection and transfer techniques minimize the risk of transmitting agents that could infect the uterus and or placenta. Rederivation by embryo transfer decreases the risk of a foster mother rejecting pups. This method eliminates the need for precise timing of cesarean surgery, a common obstacle with transgenic and knock-out lines in which gestation length often varies. Charles River performs hundreds of embryo transfer rederivations each year.


Rederivation Service Flow

  1. Charles River organizes transfer of animals to our site.
  2. Project Manager determines objectives and strategy for the project once animals are received. Results are guaranteed based on standard reproductive parameters.
  3. Technical team implements the rederivation project.
  4. ICM customer portal provides direct, two-way communication with project manager during rederivation project.
  5. Charles River arranges the shipment of animals at the end of the project or establishes a breeding colony and protocol in our facilities.


Supplemental Service

In conjunction with rederivation, it is often advisable to cryopreserve embryos and/or sperm to provide backup in the case of a future colony loss.