Custom Rat & Mouse Model Generation
Effective research depends on finding the most relevant animal model for your project. Our team can select, customize, create, and maintain the right transgenic model specific to your research by using CRISPR/Cas9 technology. Through a strategic partnership with our genomics partners, we're able to offer transgenic model creation and design to clients around the world.
Our experienced project management team, scientists, and animal care staff are available to offer guidance, develop work plans, and help overcome any challenges you may face.
Model Creation Services
CRISPR/Cas9* Genome Editing in Mice and Rats
Create and maintain the right transgenic model for your study using CRISPR/Cas9. This technology is used to knockout or modify DNA in animal models to study phenotypes and develop new treatments. Using genetic transfer into embryos or embryonic stem cells based on the needs of your project, transgenic animals can be obtained in 3 – 4 months.
RNAi Mouse Models
RNAi-mediated gene silencing makes complex genetic modifications easier to achieve more quickly and efficiently than traditional knockout technologies. The estimated timeline from project initiation to the generation of ES cell-derived RNAi mice is an estimated 24 – 30 weeks.
ES Cell Mutagenesis in Mice
Using ES cell technology, our experts inject custom embryonic stem (ES) cells directly into blastocysts, effectively bringing your in vitro research models to life.
Knockout mice are used to study gene function and validate treatments. Whether you're looking for constitutive, conditional, or RNAi knockout mice, we can deliver study-ready knockout cohorts to meet your research needs.
The ability to engineer the mouse genome has made transgenic mice a powerful tool to model human disease. All types of mutations for knockin mice are achievable, whether it’s constitutive, point mutation, or humanized mice.
Bridge the gap between your in vitro and in vivo mouse models by using ES cell, DNA, and CRISPR microinjections.
Random DNA Transgenesis in Mice
The DNA vector is injected directly into the pronuclei of zygotes, bringing your customer in vitro models to life with founders available in 3 – 4 months.
What are transgenic animal models?
Transgenic mice and rats are models that have their genomes altered to include a transgene or foreign sequence for the purpose of studying gene functions. Transgenic animals are used to study oncology, obesity, diabetes, aging, heart disease, and more.
Who does Charles River partner with for transgenic model creation?
Our relationship with key partners within the genetic engineering service industry ensures you have the resources to advance your research. We have partnered with PHENOMIN-ICS, Mirimus, and the University of Tsukuba to deliver a complete solution from in vitro and in vivo expertise to custom RNAi and CRISPR/Cas9 genome editing to create custom transgenic models.
What is CRISPR/Cas9*?
CRISPR/Cas9 technology is used to modify or knockout DNA in animal models for research studies to develop new therapies. It enables researchers to genetically engineer and study human diseases in mice and rats.
How can I see the progress of my transgenic model creation project?
Clients are able to maintain a real-time connection to their model creation projects with our Internet Colony Management (ICM™) platform. It gives researchers and laboratory personnel around-the-clock access to manage their transgenic animal colonies and data.
* CRISPR-Cas9 used under licenses to granted and pending US and international patents from The Broad Institute and ERS Genomics Limited.