RNAi-mediated Gene Silencing
Charles River offers potent, inducible, and reversible RNAi-mediated gene silencing for your gene of interest in mouse models. Our state-of-the-art microinjection services transform custom ES cells into your in vivo research model in about 12 weeks.
Advantages of RNAi Mice
Custom RNAi mice are usually available within 24 weeks of project initiation. When validated shRNAs are already available for your gene of interest, RNAi mice are available sooner.
Multiple genes may be targeted directly in embryonic stem cells, followed by mouse generation, making very complex genetic modifications possible more quickly and reducing breeding time and costs.
Controlling the timing and duration of gene suppression through a TET-inducible system allows increased flexibility in experimental design.
Expanded Gene Targets
The inducible and reversible nature of gene suppression allows genes to be manipulated in vivo.
Suppressing gene function, as opposed to a complete knockout, more closely mimics the activity of small-molecule agonists.
Timeline for RNAi Production Method
Estimated timeline: 6 – 7.5 months
- 0-month mark ►Project initiation
- 3-month mark ►Generation of potent shRNAs
- 5-month mark ►Generation and validation of target RNAi ES cell lines
- 6-month mark ►Generation of ES cell-derived RNAi mice
RNAi ES Injection
The RNAi ES technique follows the generation of potent RNAs. This material is implanted into embryonic stem cells, which are then implanted into the foster female.
- Generation of potent shRNAs
- Injection into ~ 80 embryos
- Reimplantation into VAF/Elite® foster female
- Husbandry and weaning
- VAF/Elite® health report
Delivery of chimeric mice
Includes package I services and:
- Breeding to F1 generation
- Sample collection and screening up to 40 mice
Delivery of heterozygous F1 mice
Charles River’s model creation services are enhanced by a comprehensive portfolio of support services in embryology (e.g., cryopreservation, microinjection), genetic testing and breeding that can protect the long-term integrity of valuable mouse model lines.