ES Cell, DNA & CRISPR Microinjection

Bridge the gap between your in vitro and in vivo models by accessing the microinjection services offered at Charles River. Our experienced technicians use cutting-edge equipment and techniques to prepare and inject your embryonic stem (ES) cells into blastocysts or your genetic material (e.g., CRISPR) into mouse one-cell embryos. Watch this video of a blastocyst injection.

 

Microinjection Techniques in Animals

We offer various injection techniques to help you create the right animal model for your studies.

ES CELL INJECTIONS
DNA INJECTIONS
  • Package I - Plasmid Vectors
    • Injection into one-cell embryos
    • Reimplantation into foster female
    • Husbandry and weaning
    • Biopsies for genetic testing
    • Full health report
    • Delivery of transgenic mice
  • Package II - BAC

    Includes Package I services plus breeding to F1 generation and delivery of heterozygous F1 mice

CRISPR INJECTIONS
  • Package I
    • Injection into embryos
    • Reimplantation into females
    • Husbandry and weaning
    • Biopsies for genetic testing
    • Full health report
    • Delivery of transgenic mice
  • Package II

    Includes Package I services and breeding to the F1 generation and delivery of heterozygous mice

DISCUSS YOUR PROJECT/REQUEST A QUOTE

CRISPR-Cas9 used under licenses to granted and pending US and international patents from The Broad Institute and ERS Genomics Limited.