ES Cell, DNA & CRISPR Microinjection
Bridge the gap between your in vitro and in vivo models by accessing the microinjection services offered at Charles River. Our experienced technicians use cutting-edge equipment and techniques to prepare and inject your embryonic stem (ES) cells into blastocysts or your genetic material (e.g., CRISPR) into mouse one-cell embryos. Watch this video of a blastocyst injection.
Microinjection Techniques in Animals
We offer various injection techniques to help you create the right animal model for your studies.
-
Package I
- Injection into blastocysts
- Reimplantation into foster female
- Husbandry and weaning
- Full health report
- Delivery of chimeric mice
-
Package II
Includes Package I services plus breeding to F1 generation and biopsies for genetic testing
- Technical Sheets
-
Package I - Plasmid Vectors
- Injection into one-cell embryos
- Reimplantation into foster female
- Husbandry and weaning
- Biopsies for genetic testing
- Full health report
- Delivery of transgenic mice
-
Package II - BAC
Includes Package I services plus breeding to F1 generation and delivery of heterozygous F1 mice
-
Package I
- Injection into embryos
- Reimplantation into females
- Husbandry and weaning
- Biopsies for genetic testing
- Full health report
- Delivery of transgenic mice
-
Package II
Includes Package I services and breeding to the F1 generation and delivery of heterozygous mice
DISCUSS YOUR PROJECT/REQUEST A QUOTE
CRISPR-Cas9 used under licenses to granted and pending US and international patents from The Broad Institute and ERS Genomics Limited.