Immunohistochemistry - IHC Testing
In studies of RNA abundance and gene expression, no single technique can provide all of the answers, so it’s necessary to use a combination of several methods. We have extensive experience working with IHC testing procedures as well as in situ hybridization (ISH) techniques for localizing gene expression, proteins, and mRNA.
We offer a range of validated procedures and can develop new ones to answer study-specific questions. We also have a variety of both isotopic and non-isotopic ISH testing probes for a wide range of mRNAs, and new probes can be developed as needed.
Immunohistochemistry and In Situ Hybridization Services
- Safety assessments for approximately 70 humanized, chimeric, or murine monoclonal antibodies annually
- Protocol development for over 80 different antibodies in a variety of species
- Experience with unconjugated antibodies, antibodies conjugated to a variety of substances, and Fab fragments
- Techniques to reduce or eliminate the binding of secondary reagents to endogenous immunoglobulin, even when the tissue is of the same species as the immunoglobulin
- Reproducibility of immunohistochemical techniques in conjunction with morphometric and stereological analyses
- QIHC-certified staff using state-of-the-art automated staining instruments
- Double IHC staining
- RNAscope® technology
- Fluorescein probe
- Chromogenic probe
- Biotin probe
- Digoxigenin probe
- 33P-labeled probes
- Autoradiographic emulsion detection
- Microautoradiography detection
Optimizing IHC Staining through Automation
Our Thermo Scientific™ Lab Vision™ Autostainer 720 saves time, decreases variation, and increases efficiency for all your immunohistochemistry needs. Learn How it Works
When we pair our in situ hybridization methods with cell type-specific immunostaining, we can generate a more complete picture of mRNA and protein expression within specific cell types in tissue sections. These ISH assays can be used to assess tissue responses to various drug treatments for proof-of-principle and/or efficacy and safety evaluations.
Frequently Asked Questions (FAQs) about Immunohistochemistry and In Situ Hybridization
What is the difference between immunohistochemistry and in situ hybridization?
Immunohistochemistry is the detection of a specific antigen (typically a protein) in thin tissue sections or cells mounted on slides for microscopic evaluation. Immunohistochemistry is the detection of a protein of interest in thin tissue sections or cells mounted on slides for microscopic evaluation. In situ hybridization is also done on thin tissue sections or cells mounted on slides, but it detects a specific sequence or region of DNA or RNA.
What is immunohistochemistry used for?
It is used when spatial and morphological context of the antigen is desirable. During the preclinical development of novel therapeutics, it is commonly used to assess efficacy or toxicity in vivo. In a clinical setting, it can be used for diagnostic purposes.
What makes our services stand out from the competition?
We can work with you closely to develop an immunohistochemistry or immunofluorescence assay specific to your needs. Our scientists have the experience and knowledge needed to understand the nuances of complex projects. Our staining process is automated, resulting in enhanced throughput and consistency.