Comprehensive Molecular Pathology Techniques

Immunohistochemistry and In Situ Hybridization Services

We assist clients with their study-specific questions through a range of validated molecular pathology procedures. With extensive experience working with immunohistochemistry (IHC) and in situ hybridization (ISH) techniques, our team of industry experts can even develop new protocols as needed.

Further, we can provide clients with tissue microarrays for high-throughput tissue screening of IHC and ISH staining. The results can be evaluated by one of our expert pathologists or quantified by automated image analysis.

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Optimizing IHC Staining through Automation

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Immunohistochemistry and In Situ Hybridization Capabilities

    • Safety assessments for approximately 70 humanized, chimeric, or murine monoclonal antibodies annually
    • Protocol development for over 80 different antibodies in a variety of species
    • Experience with unconjugated antibodies, antibodies conjugated to a variety of substances, and Fab fragments
    • Techniques to reduce or eliminate the binding of secondary reagents to endogenous immunoglobulin, even when the tissue is of the same species as the immunoglobulin
    • Reproducibility of immunohistochemical techniques in conjunction with morphometric and stereological analyses
    • QIHC-certified staff using state-of-the-art automated staining instruments
    • Double IHC staining
    • RNAscope® technology
    • Fluorescein probe
    • Chromogenic probe
    • Biotin probe
    • Digoxigenin probe
    • 33P-labeled probes
    • Autoradiographic emulsion detection
    • Microautoradiography detection

In studies of RNA abundance and gene expression, no single technique can provide all the answers. When we pair our ISH methods with cell type-specific immunostaining, our team can generate a more complete picture of mRNA and protein expression within specific cell types in tissue sections. These ISH assays can be used to assess tissue responses to various drug treatments for proof-of-principle and/or efficacy and safety evaluations.

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Frequently Asked Questions (FAQs) about Immunohistochemistry and In Situ Hybridization

  • What is the difference between immunohistochemistry and in situ hybridization?

    Immunohistochemistry is the detection of a specific antigen (typically a protein) in thin tissue sections or cells mounted on slides for microscopic evaluation. Immunohistochemistry is the detection of a protein of interest in thin tissue sections or cells mounted on slides for microscopic evaluation. In situ hybridization is also done on thin tissue sections or cells mounted on slides, but it detects a specific sequence or region of DNA or RNA.

  • What is immunohistochemistry used for?

    It is used when spatial and morphological context of the antigen is desirable. During the preclinical development of novel therapeutics, it is commonly used to assess efficacy or toxicity in vivo. In a clinical setting, it can be used for diagnostic purposes.

  • What makes our services stand out from the competition?

    We can work with you closely to develop an immunohistochemistry or immunofluorescence assay specific to your needs. Our scientists have the experience and knowledge needed to understand the nuances of complex projects. Our staining process is automated, resulting in enhanced throughput and consistency.

  • What is a tissue microarray?

    A tissue microarray (TMA) consists of multiple small “cores” of tissues on a single microscope slide. Tissue cores are taken from “donor” tissue blocks, which are optimally fixed and preserved tissues taken from healthy animals. TMA blocks can be completely customized with regard to species, tissue types, size, and number of cores.

  • What is tissue microarray technology used for?

    Tissue microarray (TMA) slides allow for rapid, high-throughput screening of molecular markers across numerous tissues. It is an efficient, cost-effective way to determine what tissues contain the protein (for IHC) or RNA (for ISH) of interest. TMA slides are also utilized for screening of antibodies during IHC optimization procedures. In this case, tissues are often combined with cell pellets to test specificity and sensitivity of the antibody being tested.