Ocular Pathology Services

Key areas of our expertise in ocular pathology include:

  • Immunohistochemistry
    • Immunohistochemistry methods allow for the visualization and morphometric analysis of implanted or transformed cells in ocular target tissues.
    • These assays can be used to assess tissue responses to various drug treatments for proof-of-principle and efficacy as well as safety evaluations.
    • We offer a wide range of validated IHC procedures and can develop new ones to answer study-specific questions.
  • Polymerase Chain Reaction (PCR)
    • PCR is an extremely sensitive and specific fluorescence technology that provides rapid quantitative results on the distribution (qPCR) and expression (RT-qPCR) of target DNA or RNA.
    • GLP-compliant molecular pathology services at Charles River (both PCR-based and in situ hybridization evaluations) allow exact identification of cells in tissues.
    • The integration of molecular biology and histopathology capabilities (as described below) allows for the correlation of gene expression to tissue histomorphology, which provides valuable functional genomics information.
  • Specular Microscopy
    • A specular microscope allows for the noninvasive in vivo evaluation of the corneal endothelium. Damage to this layer can rapidly result in corneal edema and visual deficiency caused by reduced refraction.
    • Microscopy uses a focused beam of electrons instead of light to examine the eye at a very granular level. Recognized for our experience in this field, we provide non-GLP as well as GLP-compliant microscopy with both scanning and transmission capabilities.