In studies of RNA abundance and gene expression, no one technique can provide all of the answers, so it is necessary to use a combination of several methods. Charles River has extensive experience working with immunohistochemical (IHC) procedures as well as in situ hybridization (ISH) techniques for localizing gene expression, proteins, and mRNA.
When we pair our ISH methods with cell-type-specific immunostaining, we can generate a more complete picture of mRNA and protein expression within specific cell types in tissue sections. These assays can be used to assess tissue responses to various drug treatments for proof-of-principle and/or safety and efficacy evaluations.
We offer a range of validated IHC procedures and can develop new ones to answer study-specific questions. We also have a variety of both isotopic and non-isotopic ISH probes for a wide range of mRNAs, and new probes can be developed as needed.
- Safety assessments for approximately 70 humanized, chimeric or murine monoclonal antibodies annually
- Protocol development for over 80 different antibodies in a variety of species
- Experience with unconjugated antibodies, antibodies conjugated to a variety of substances and Fab fragments
- Techniques to reduce or eliminate the binding of secondary reagents to endogenous immunoglobulin, even when the tissue is of the same species as the immunoglobulin
- Reproducibility of immunohistochemical techniques in conjunction with morphometric and stereological analyses
- QIHC-certified staff using state-of-the-art automated staining instruments
- Double IHC staining
- RNAscope® technology
- Fluorescein probe
- Chromogenic probe
- Biotin probe
- Digoxigenin probe
- 33P-labeled probes
- Autoradiographic emulsion detection
- Microautoradiography detection