Overview

Cardiac action potential duration (APD) assays are recommended for candidate selection and Investigational New Drug (IND) submissions.

APD SC-derived Human Cardiomyocytes

Manual patch clamp recordings of action potentials in isolated induced pluripotent stem cell-derived human cardiomyocytes (SC-hCMs) evaluate paced intracellular electrical response for detection of drug-induced action potential prolongation and arrhythmia.

Benefits

  • Detection of action potential prolongation (QT risk) and EADs (trigger for torsades de pointes)
  • Stable recordings at physiological temperature with minimal diffusion delays
  • Human test system

APD Purkinje Fiber

Intracellular recordings of action potentials in animal Purkinje fibers evaluate paced electrical response for detection of drug-induced action potential prolongation and arrhythmia.

Benefits

  • The APD assay, together with the hERG assay, is an effective indicator of multiple ion channel effects (MICE).
  • Assays available as GLP or non-GLP