Comet Assay Studies

In vivo comet assays are primarily used for substances with positive in vitro mutagenicity test results and/or for mechanistic studies. Our in vitro version of this assay in a 3D skin tissue model can help replace animal testing according to official OECD guidelines. In vivo comet assay techniques can be applied to any species and organ from which a single-cell suspension can be prepared. See which comet assay is best to use in your genetic toxicology study.

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In Vivo Comet Assay

When determining if a compound is genotoxic, the comet assay (also known as the single-cell gel electrophoresis [SCGE] assay) helps to evaluate DNA strand breaks in individual cells. A single-cell suspension from the selected tissue(s) is embedded in low melting point agarose, lysed, and electrophoresed under alkaline (pH > 13) conditions. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often looks like a celestial comet.

In Vivo Comet Assay

Image of FT Skin Models full-thickness, reconstructed 3D skin model histology.

The 3D Skin Comet Assay

Phenion® FT Skin Models are full-thickness, reconstructed 3D skin models comprised by the two main layers of human skin – epidermis and dermis. These models retain full epidermal differentiation as observed in human skin, including stratum basal, spinosum, granulosum, lucidum, and corneum. This 3D skin comet assay may serve as a potential in vitro alternative to animal testing.
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