Reverse transcription quantitative PCR (RT-qPCR) is often the method of choice for performing gene expression assessments with alternative methodologies including branched DNA assays (QuantiGene), NanoString, or droplet digital PCR (ddPCR). This poster presentation outlines the simple and efficient workflow for validating RT-qPCR assays for mRNA quantification using a single-step, DNA standard curve-based RT-qPCR method.

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