CRISPR/Cas9 has revolutionized the creation of complex mouse and rat models. Genome editing opportunities can seem infinite, but there are parameters that must be considered.

This webinar presents how we've generated most of the desired structural variant and CNV rearrangements - achieving deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rodents (the good). However, we must also consider limitations of modifying “big” genomic sequences (the bad) and how precise validation of the lines generated by CRISPR/Cas9 is demanded (the ugly).


  • Guillaume Pavlovic, PhD, Head of the Genetic Engineering and Model Validation Department, PHENOMIN-ICS
  • Jean Cozzi, PhD, Embryology Laboratory Manager, Charles River