Overview

A single intrarectal instillation of haptenizing molecule 2,4,6-trinitrobenzene sulfonic acid (TNBS) in vehicle ethanol is known to induce immunogenic reactions in the colon wall and generate colitis in mice that models the human condition. CD4+ T cells are suggested to play a key role in inducing colitis in this mouse model. The acute TNBS colitis model is usually induced by a single intrarectal administration of TNBS and then observed for 5-7 days.

How can we support your program?

Histopathologically, the TNBS model is characterized by more focal damage in the colon than the DSS colitis model. Presensitization may be used to mimic a delayed type hypersensitivity reaction. Our inflammation group has validated this model for use in testing efficacy of novel therapeutic compounds.

  • VALIDATION DATA
    chart of body weight loss in 2% TNBS-treated mice compared with vehicle ethanol-treated mice

    Body weight loss in 2% TNBS-treated mice compared with vehicle ethanol-treated mice.

     

    H&E stained section of colon from TNBS-treated mice

    H&E stained section of colon from TNBS-treated mice.

  • STUDY ENDPOINTS
    • PK/PD blood collections
    • Cytokine/chemokine analysis
    • Clinical chemistry
    • Histopathological evaluation
    • Immunohistochemistry
    • Myeloperoxidase (MPO) activity assay